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Research ArticleOpen accessIn the keywordsResidues Depletion Study and Withdrawal Period Determination of Sulphadiazine and Trimethoprim Premix in PigsPanagiotis Dimitrios Katsoulos, Lydia Dritsa, Georgios Christodoulopoulos et al.The emergence and spread of antibiotic resistant human pathogens have been directly linked to the use of antibiotics in livestock production. The purpose of this study was to detect and quantify the concentration of the residues of Sulphadiazine (SDZ) and Trimethoprim (TMP) in edible tissues of pigs and to determine the withdrawal period after oral administration of OPTIPRIME® premix 40%, … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 1 · 12 Feb 2013 · 8,360 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEmerging Trends in Designing Short and Efficient Protein Purification ProtocolsSaurabh Gautam, Joyeeta Mukherjee, Ipsita Roy, Munishwar Nath GuptaProtein purification is carried out in both academic and industrial sectors. Both non-chromatographic approaches (precipitation, crystallization, partitioning) and chromatographic methods are used. When recombinant proteins are over expressed in foreign hosts, inclusion bodies are obtained. Many purification methods can be used to refold proteins. Many strategies allow one to avoid formation of inclusion bodies. The concept of affinity based separation … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,007 accessesView PDFCite
Research ArticleOpen accessIn the keywordsFORMULATION AND CLINICAL TESTING OF GLYCERYL TRINITRATE OINTMENT FOR TREATMENT OF ANAL FISSURESEmad Mothana Yousif, Harith Jameel Mahdi, Ali Ibrahem Khaleel, Shathel K. NoamanUntil know surgical intervention considered to be the cornerstone for treatment of anal fissure condition. Regarding all the circumstances that hinder the possibility of accomplishment of surgical operation and the possible post-urgical complications; all that motivates the necessity for a non-surgical procedure for treatment of anal fissure. This study was designed to establish a pharmaceutically active and chemically and physically … …American Journal of Pharmacology and Toxicology, Vol. 7, Issue 3 · 1 Sep 2012 · 8,965 accessesView PDFCite
Research ArticleOpen accessIn the keywordsImplications of Method Chosen for Analysis of Fatty Acids in Meat: A ReviewLivia Facuri Araujo Macedo, Ellen Cristina Quirino Lacerda, Roberio Rodrigues Silva et al.The influence of the beef Fatty Acid (FA) profile on human health, has become a recurring concern. For an analysis of the lipid composition of food, it is necessary that the complex lipids be quantified in a suitable form for subsequent analysis of their composition by gas chromatography. The choice of appropriate methods of lipid extraction and esterification of fatty … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 3 · 9 Jul 2012 · 8,633 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPhysico-Chemical Characteristics of Date Seed Oil Grown in SudanRehab Salih Mahmoud Abdalla, Alsheikh AlGilani Albasheer, Abdel Rahim Mohamed ELHussein, ELrasheed Ahmed GadkariemProblem statement: Studies were conducted on the physical-chemical properties of Sudanese date seed oil extracted from two date palm cultivars (Phoenix dactylifera L.) ALBarakawi and Alqundeila. The extracted oil from ALBarakawi seeds weighed 6.833% w/w oil, Alqundeila oil extracted weighed 5.064% w/w. Approach: The physical properties of ALBarakawi oil were: viscosity: 17cp, refractive Index: 1.444, density: 0.9116 g cm-3 color: … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 5 May 2012 · 9,500 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRole of Evaporation in Degrading the "Oil Lakes"Al Rashed Ahamad, Colin BarkerProblem statement: Oil Spills are degraded by aerobic bacteria, water washing, evaporation and oxidation. In the absence of water the first two of these processes cannot operate. This is the situation in the low rainfall environment of the Kuwaiti desert where oil lakes still persist 20 years after the initial spills. The oils contain longer chain n-alkanes but have lost … …American Journal of Environmental Sciences, Vol. 7, Issue 3 · 11 Jul 2011 · 6,747 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,329 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIdentification of Volatile Metabolites from Fungal Endophytes with Biocontrol Potential towards Fusarium oxysporum F. sp. cubense Race 4A.S.Y. Ting, S.W. Mah, C.S. TeeProblem statement: Fungal endophytes are widely studied for their potential as biocontrol agents towards fungal pathogens. In vitro assessments usually reveal their antibiosis and mycoparasitism nature, but little is understood regarding their production of volatile metabolites as mechanisms of antagonism. Approach: This study explored the potential of fungal endophytes in controlling the pathogen responsible for Fusarium wilt disease. Nine fungal … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 2 · 30 Jun 2010 · 11,872 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHeroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA)Jessica L. Reynolds, Supriya D. Mahajan, Donald Sykes, Madhavan P.N. NairHeroin use is postulated to act as a cofactor in the neuropathogenesis of human immunodeficiency virus (HIV-1) infection. Astrocytes, integral components of the CNS, are reported to be susceptible to HIV-1 infection. Upon activation, astrocytes release a number of immunoregulatory products or modulate the expression of a number of proteins that foster the immunopathogenesis of HIV-1 infection. However, the role … …American Journal of Infectious Diseases, Vol. 2, Issue 2 · 30 Jun 2006 · 7,261 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite