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45 articles for “Yuanda Song”
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Research ArticleOpen accessIn the author namesInhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitroShuqing Yang, Haifang Xiao, Shuyan Yu et al.In this study, the effect of Walnut Leaf Polysaccharides (WLP) on human liver tumor HepG2 cells was investigated. The results showed that WLP had dose-dependent and time-dependent inhibitory effects on HepG2 cell viability. Typical apoptosis characteristics were observed in WLP-treated HepG2 cells through 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl-carbocyanine iodide (DAPI) staining and Acridine Orange (AO)/Ethidium Bromide (EB) dual staining. Meanwhile, significant decrease in Mitochondrial … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 29 Oct 2019 · 7,313 accessesView PDFCite
Research ArticleOpen accessIn the author namesOptimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in VitroShuqing Yang, Haifang Xiao, Shuyan Yu et al.Cellulase-assisted extraction of Walnut Leaf Polysaccharide (WLP) was studied. Single factor test and response surface design were employed to optimize the technological conditions. The consequences indicated that the highest WLP yield of 5.21% was achieved with raw material ratio of 50 mL/g, cellulase dose of 41488.52 U/g, enzymolysis pH of 5.88, enzymolysis time of 29.74 min and enzymolysis temperature of … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 23 Oct 2019 · 7,450 accessesView PDFCite
Research ArticleOpen accessIn the author namesImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDFCite
Research ArticleOpen accessIn the author namesHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Research ArticleOpen accessIn the author namesResponse Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from Walnut Leaves and In Vitro Antibacterial ActivitiesYuanda Song, Aydin Berenjian, Han Wu et al.In this paper, the optimum extraction conditions of total flavonoids extracted from walnut leaves subjected to Ultrasonic Assisted Extraction (UAE) were optimized by Response Surface Methodology (RSM). The mathematical model showed the high coefficient of measurement (R2 = 0.9938) which indicated that this model could be used to guide the response surface methodology. The optimum extraction parameters for extracting flavonoids … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 24 Oct 2017 · 7,825 accessesView PDFCite
Research ArticleOpen accessIn the author namesEnhanced Fatty Acid Production in Escherichia coli by Over-Expression of NADPH Generating EnzymesYuanda Song, Yong Q. Chen, Haiqin Chen et al.NADPH is an essential factor for Fatty Acid (FA) biosynthesis. The effect of NADPH generating enzymes [NADP+-dependent malic enzyme (NADP-ME), NADP+-dependent isocitrate dehydrogenase (NADP-IDH), Glucose 6-Phosphate Dehydrogenase (GPD) and 6-Phosphogluconate Dehydrogenase (PGD)] on FA biosynthesis was investigated in an engineered Escherichia coli BL21 △fadE/pTE. Among NADPH-generating enzymes, GPD and ICD increased total FA production by 60 and 16% respectively. Especially … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Oct 2017 · 8,578 accessesView PDFCite
Research ArticleOpen accessIn the author namesNutritional and Fatty Acids Profile Analyses of Commonly Consumed Fresh Water Fish Species in PakistanAhsan Hameed, Syed Ammar Hussain, Muhammed Asim Shabbir et al.The aim of this study is to evaluate nutritional value of selected commonly consumed fresh water fish species of Pakistan. Proximate composition and fatty acids profile of mostly consumed fresh water fish species of Pakistan namely Balm or Baam (Anguilla anguilla), Sole (Channa marulius), Damra (Labeo rohita) and Malhi (Wallago atto) were analysed. Proximate composition data showed a significant difference … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 1 · 31 Jan 2017 · 9,515 accessesView PDFCite
Research ArticleOpen accessIn the author namesCocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation TechnologyLina Zhao, Bo Li, Dan Xiong et al.In order to obtain the optimal control point for single-cell lipid production with fatty acid profile similar to cocoa butter, this study explored the effects of carbon source, nitrogen source, temperature, sterculic acid methyl ester, cobalt on cell growth and lipid accumulation of Yarrowia lipolytica. Glycerol and ammonium tartrate-yeast extract were chosen as carbon source and nitrogen source for production … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 29 Dec 2016 · 9,285 accessesView PDFCite
Research ArticleOpen accessIn the author namesIdentification and Characterization of Diacylglycerol Acyltransferase in Oleaginous Yeast Rhodosporidium toruloidesZhen Wang, Huaiyuan Zhang, Lina Zhao, Yuanda SongDiacylglycerol acyltransferase (DGAT), which catalyzes TAG formation from DAG and acyl-CoA, has been considered to play a vital role in TAG accumulation in oleaginous microorganisms. The genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which shared little conserved amino acid coding sequence with each other. Phylogeny tree analysis showed that RtDGATa belonged to DGAT1 … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,387 accessesView PDFCite
Research ArticleOpen accessIn the author namesBinding Interaction between Bovine Serum Albumin and Chicoric Acid, a Food Functional ComponentHaifang Xiao, Quancai Sun, Xuebo Liu, Yuanda SongFuorescence, FTIR and UV-Vis absorption spectroscopy were used to explore the binding between chicoric acid and Bovine Serum Albumin (BSA). Binding characteristics at various levels of temperature have been calculated. The results indicated that chicoric acid statically quenched the intrinsic fluorescence of BSA. The binding constants (Ka) were 4.14×105 L mol-1 at 273K and 4.29×106 L mol-1 at 298 k. … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 19 Sep 2016 · 7,552 accessesView PDFCite