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44 results for “Molecular Detection”
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Research ArticleOpen accessIn the abstractPRODUCTION AND CHARACTERIZATION OF MONOCLONAL AND POLYCLONAL ANTIBODY AGAINST RECOMBINANT OUTER MEMBRANE PROTEINMahdi Fasihi-Ramandi, Amir Nedjad-Moghaddam, Fatemeh Arabsalmany et al.There are many studies related to immunological and molecular methods for diagnosis of Vibrio cholera (V. cholerae). However, most assays dependent on enrichment of culture of bacteria, which need more time and involves the use of costly equipment and reagents. In this study Balb/c mice were immunized with recombinant Outer Membrane Protein (rOMPw) of vibrio cholerae and splenocytes of hyper … …American Journal of Immunology, Vol. 10, Issue 1 · 26 Apr 2014 · 7,439 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Certain Tetracyclines and Oxytetracyclines through Charge Transfer ComplexationKhairi M.S. FahelelbomProblem statement: Several studies were carried out to develop and validate a simple, rapid and accurate spectrophotometric method for the analysis of tetracycline (TC) and oxytetracycline (OTC) in the pure form and in pharmaceutical dosage forms, despite the varieties of these analytical techniques, the literature revealed that no spectrophotometric method for determination of tetracycline and oxytetracycline using charge transfer complexation … …American Journal of Pharmacology and Toxicology, Vol. 3, Issue 3 · 30 Sep 2008 · 8,528 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmunohistochemistry Assay to Detect Turkey Coronavirus (TCoV) from Experimentally Infected PoultsThais Larissa L Castanheira, Heitor F. Ferrari, Maria Cecília R. Luvizotto, Tereza C. CardosoThe objective of this study was to develop a direct immunohistochemical assay to detect TCoV antigens in formalin-fixed paraffin-embedded sections prepared from experimentally infected poults. The sections of ileo, ileo-cecal junction and ceca regions from intestine were prepared and submitted to two different primary antibodies, first the non-biotin labeled polyclonal antibody for the indirect method, and second the biotin-labeled polyclonal … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,900 accessesView PDFCite
Research ArticleOpen accessIn the abstractNa,K-ATPase α Subunit-like Immunoreactive Proteins in Human Body FluidsJeng-Hsiung F. PengNa,K-ATPase was recently considered a target for breast cancer treatment since its potent inhibitors ouabain and related digitalis compounds that were reported circulating in body fluids before, were found to possess potent anti-breast cancer activity. Based on this finding, it is hypothesized that Na,K-ATPase derivatives could be circulating in the body and potential candidate biomarkers for early disease detection. Western … …American Journal of Immunology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,892 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordEffects of Aerobic Exercise on PPAR-γ Expression in Adipose Tissue and RT-PCR Detection AnalysisXiaodong Zhan, Chenguang Bi, Jie WeiThis study aimed to examine the effect of organized aerobic exercise on the expression level of Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) gene in adipose tissue by performing digital variant of RT-PCR, Droplet Digital PCR (ddPCR) which is a highly sensitive method for counting the gene copy numbers. The proposed scheme consists of three-stage analytical procedure, namely, Subphase I Controlled Aerobic … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 29 Sep 2026 · 580 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordEarly Detection of Endobacteria in Rhizopus spp.Ratna Noviyanti, Gayuh Rahayu, Rida Oktorida KhastiniRhizopus spp. are commonly used in bio-industrial processes such as manufacturing traditional fermented foods. However, one species, R. microsporus has been reported to contain toxin-producing endobacteria. Therefore, it is essential to evaluate the Rhizopus strains' safety before application as tempe inoculum. The study aimed to detect the presence of endobacteria from seven strains of Rhizopus spp. isolated from Tempe and … …OnLine Journal of Biological Sciences, Vol. 23, Issue 3 · 24 Jun 2023 · 5,331 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordMolecular Identification of Domestic and Wild Boars Using Cytochrome-b Specific Primers for Halal AuthenticationRifqi, Edi Suryanto, Ari Surya Sukarno et al.The subject of adulterating wild boar and pig meat products with other animal meat is delicate, particularly in the Muslim nation of Indonesia. In recent years, there has been a lot of concern over halal meat products that contain wild boar and pig meat due to Economically Motivated Adulteration (EMA). However, there are limited studies of PCR-DNA-based to discriminate between … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 3 · 14 Nov 2025 · 2,378 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordParticipation of Dermacentor Reticulatus Imago in the Reservation of Bovine Leukemia VirusY. Glazunov, Ya. Kabitskaya, L. Glazunova et al.Strict implementation of measures for the prevention and elimination of bovine leukemia does not allow completely getting rid of this disease. In all likelihood, the leukemia virus has additional transmission routes that have not been taken into account until today. In the Tyumen region, blood-sucking insects and ticks are widespread and take part in the transmission of some infectious and … …OnLine Journal of Biological Sciences, Vol. 22, Issue 4 · 2 Nov 2022 · 5,074 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordMolecular Characterization of Vaccine-Derived Mutants of Avian Metapneumoviruses Isolated from Turkeys in EgyptAbdel-Satar Arafa, Wessam Mady, Ahmed Hussein et al.Avian metapneumovirus (aMPV) causes acute respiratory infection in turkeys with sudden onset and rapid spread in the infected flocks. Vaccinated flocks frequently showed clinical signs typical like the non-vaccinated flocks in Egypt, this situation suggests a virulent vaccine virus was circulating in the field. Oropharyngeal and nasal sinus swabs were collected from 100 turkeys from 10 commercial farms with mild … …Current Research in Virology, Vol. 4, Issue 1 · 12 Aug 2015 · 12,748 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordDetection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism MethodHossein Ali Abbasi, Mohsen KazemiProblem statement: Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in chickens. The Insulin like Growth Factor-I (IGF1) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. Approach: In the present study polymorphism of the promoter and 5' untranslated region of IGF-1 gene of Mazandaran … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 8 Aug 2011 · 7,627 accessesView PDFCite