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44 results for “Molecular Detection”

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Research ArticleOpen accessPhrase in the titleMolecular Detection of Follicle Stimulating Hormone Receptor Polymorphisms and Luteinizing Hormone Gene Variations in Primary and Secondary Iraqi Infertile WomenNoor Basim Naser, Dr. Ezeddin Atia Albayyar, Dr. Susan Abed ZaidanThe current study aimed to determine the role of hormonal disturbances in infertility, moreover assessing variations at the molecular level that could affect this disease. The study examined 90 blood samples, 30 patients with primary infertility, 30 patients with secondary infertility and 30 healthy women as control. FSH and LH were measured using Electrochemiluminescence assay. DNA was isolated for molecular … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 15 Nov 2018 · 7,553 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleISOLATION AND DETECTION OF HELICOBACTER PYLORI FROM PATIENTS SUFFERING FROM PEPTIC ULCER USING BIOCHEMICAL TESTS AND MOLECULAR TECHNIQUESRuba S. Abu-Sbeih, Azmi D. Hawari, Dhia S. Hassawi, Hala I. Al-DaghistaniHelicobacter pylori is the causative agent of most cases of gastritis. There is no established gold standard for the diagnosis of H. pylori infection. A reliable diagnosis is crucial to confirm that eradication therapy has been successful. Eighty gastric biopsy and blood samples were obtained from fasting Jordanian patients with Esophago-Gastro-Doudenoscopy (EGD). Several diagnosis tests for H. pylori infections were … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Mar 2014 · 8,183 accessesView PDFCite
Research ArticleOpen accessIn the abstractAlpha Thalassemia Carrier Detection a Diagnostic ApproachH. R. Samruddhi, Anupama Hegde, Harsha Prasada L et al.Alpha-thalassemia is one of the most common single-gene disorders, and its carrier state often remains undetected due to nonspecific hematological findings. This study aimed to identify alpha-thalassemia carrier status among people being investigated for hemoglobinopathy in a tertiary care setting. A cross-sectional laboratory-based study was conducted from January 2023 to January 2024. Hemoglobin variant analysis reports by High Performance Liquid … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 22 Sep 2026 · 481 accessesView PDFCite
Research ArticleOpen accessIn the abstractCytotoxicity Evaluation of Curcumin-FITC Conjugates in Granulosa Cells Isolated from Ovarian Follicles of Kampung Chicken (Gallus gallus domesticus)Sukarman Hadi Jaya Putra, Mansur S, UmarudinLinking curcumin to fluorescein isothiocyanate (FITC) results in a hybrid fluorescent marker (curcumin-FITC) that exhibits improved stability and sensitivity in detection. This study sought to evaluate the biological safety and cytotoxic effects of curcumin-FITC on granulosa cells obtained from ovarian follicles (2-5 mm, F1-F4 stage) of Kampung chickens. Following fluorescent labeling with different concentrations of curcumin-FITC (0-100 μg/mL), cell viability … …OnLine Journal of Biological Sciences, Vol. 26, Issue 2 · 14 May 2026 · 1,497 accessesView PDFCite
Research ArticleOpen accessIn the abstractCo-infestation of Honey Bee Colonies with Tropilaelaps mercedesae and Varroa destructor in the Republic of KazakhstanUlzhan Nuraliyeva, Gaukhar Moldakhmetova, Oleg Krupskiy et al.Beekeeping is a well-developed industry in the Republic of Kazakhstan; however, significant winter colony losses necessitate the annual import of thousands of honey bee package colonies from neighboring southern countries. The recent detection of the invasive mite Tropilaelaps mercedesae in several of these source countries poses a serious biosecurity threat to Kazakhstani apiculture. This study aimed to identify potentially infested … …American Journal of Animal and Veterinary Sciences, Vol. 21, Issue 1 · 11 Apr 2026 · 1,850 accessesView PDFCite
Review ArticleOpen accessIn the abstractMachine Learning Advancements for Lung Cancer Detection: An In-Depth Review and Future ProspectsAanchal Vij, Kuldeep Singh Kaswan, Anand NayyarLung cancer is a major cause of cancer-related mortality globally, underscoring the necessity for efficient diagnostic instruments. The review paper summarizes the role of ML and DL in detection, staging, and prognostication of lung cancer. We evaluate the relative efficacy of several models, such as CNNs, SVMs, and ensemble approaches, by analyzing publically accessible imaging and molecular information. We emphasize … …Journal of Computer Science, Vol. 22, Issue 1 · 1 Mar 2026 · 1,794 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Diarrhea-Causing Pathogens in South Korea Analyzed Using Molecular Diagnostic MethodsYonghee Lee, Jae Kyung KimGastrointestinal inflammation remains a prevalent global health concern. Prompt and accurate diagnosis of the condition is required to mitigate health risks in immunocompromised individuals and alleviate the economic burden linked to treatment and pathogen isolation. Challenges posed by traditional culture tests, such as long detection time and inability to isolate low-abundance organisms, can be overcome using molecular diagnostic methods, which … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 25 Jul 2024 · 5,005 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Observational Study on the Prevalence of Sexually Transmitted Infections in Cheonan from 2017 to 2021Sun Jung Lee, Tae Su Jang, Jae Kyung KimAlthough the global incidence of sexually transmitted infections is increasing annually, there are few studies in Cheonan that have analyzed the frequency of infection by individual bacterial species by age since 2017. Therefore, in this observational study, we investigated trends in sexually transmitted infections in Cheonan, South Korea, since 2017. From January 2017 to November 2021, 3,297 specimens were obtained … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 17 Apr 2024 · 6,316 accessesView PDFCite
Research ArticleOpen accessIn the abstractPCR Diagnosis for the Identification of the Virulent Gene of Salmonella in Poultry MeatD. K. Zhanabayeva, A. Y. Paritova, G. K. Murzakaeva et al.The purpose of this study was to conduct microbiological studies of poultry meat imported into Mongolia and establish the serotypes and plasmids of Salmonella and determine the virulence of the gene encoding antibiotic resistance and its transmigratory properties. The PCR molecular biological method was used and according to the results, Salmonella enteritidis was identified in chicken carcasses of the American … …OnLine Journal of Biological Sciences, Vol. 21, Issue 3 · 27 Aug 2021 · 6,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDFCite