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83 articles for “purification”
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Research ArticleOpen accessIn the keywordsEvaluation of Antioxidant and Antibacterial Activities of the Polysaccharides from Mycelium and the Culture Medium of Pleurotus Eryngii Strain UN-1Qing Liu, Haodong Wu, Ying Wu et al.Pleurotus eryngii was one of the most precious and ordinary edible mushrooms, and its polysaccharides were the major active substances. In this study, P. eryngii strain UN-1 was cultured in a liquid medium at 25℃ under a shaking of 80 rpm for 7 days. Two crude polysaccharides named CPN and CPNM were obtained from the mycelium of P. eryngii UN-1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 19 Jul 2022 · 6,176 accessesView PDFCite
Research ArticleOpen accessIn the keywordsComparison of Radial and Axial Flow Chromatography for Monoclonal Antibody Downstream Processing at Bench and Pilot ScalesAli Demirci, Frank Leu, F. James BaileyAxial Flow Chromatography (AFC) is widely used for the purification of therapeutic Monoclonal Antibodies (MAbs). However, AFC columns can generate high pressure drops across the resin bed, preventing operation of the column at high flow rates especially at pilot or manufacturing-scales. Radial Flow Chromatography (RFC) was developed to provide lower pressure drops during chromatographic separations. In these studies, small and … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,508 accessesView PDFCite
Research ArticleOpen accessIn the keywordsImpact of Abattoir and Saw-Mill Effluents on Water Quality of Upper Ogun River (Abeokuta)A. O. Adeogun, A. V. Chukwuka, O. R. IborProblem statement: The unregulated discharge of contaminants from point sources into inland freshwater aquatic systems and the resultant deterioration of water quality are largely undocumented in developing countries like Nigeria especially with respect to the role of seasonal factors on the recovery capacity of aquatic systems. Approach: The aim of this study was to assess the impact of abattoir and … …American Journal of Environmental Sciences, Vol. 7, Issue 6 · 6 Jan 2012 · 8,634 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSimple Method of Purifying Humic Acids Isolated from Tropical Hemists (Peat Soil)Susilawati Kasim, Osumanu Haruna Ahmed, Nik Muhamad Ab. Majid, Mohd Khanif YusopThe objectives of this study were: To investigate whether a purification period of HA isolated from Hemists peat soils could be reduced and to investigate whether distilled water could be used to purify HA isolated from Hemists peat soils. Standard procedures were used to extract and fractionate HA in a Hemists peat soil. The isolated HA was purified by suspending … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 7,151 accessesView PDFCite
Research ArticleOpen accessIn the keywordsScreening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicumGailing Wang, Chendi Li, Yanan Guo et al.The slow degradation of returned straw adversely affects seedling survival rate. To shorten the degradation time of lignin in maize straw in warm temperate regions, a cellulolytic microbial strain that can secrete polymer with high enzymatic activity was isolated from the humus, which helped to identify an effective strain to expedite the decomposition of returned maize straw. Using the molecular … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 23 May 2023 · 5,300 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBiochemical Studies of Lipase from Germinating Oil Seeds (Glycine max)P. P. Gadge, S. D. Madhikar, J. N. Yewle et al.Problem statement: Lipase is one of the important enzymes in food, pharmaceutical, detergent and biofuels industries. Search for the lipase with distinct features, possibly from germinating seeds, is of interest for industrial applications. Approach: The lipase produced by soybean oil seeds was partially purified and characterized in terms of the optimal pH and temperature for activity as well as substrate … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 19 Jan 2012 · 9,703 accessesView PDFCite
Research ArticleOpen accessIn the keywordsUse of a Mixture of Thermophilic Enzymes Produced by the Fungus Thermoascus aurantiacus to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse CelluloseJ. R. Monte, W. Carvalho, A. M. F. MilagresProblem statement: The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes were shown to be … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 9,212 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAlteration of the Aquifer Water in Hyperarid Climate, by Wastewater: Cases of Groundwater from Ouargla (Northern Sahara, Algeria)Djidel Mohamed, Bousnoubra-Kherici Houria, Kherici Nacer, Nezli Imed-EddineThe present study is registered, on the area of Ouargla (the Sahara septentrional, Algeria) reports of the aquifer contamination by the anarchistic urban rejections. By its geomorphology (basin), its hyperarid climate and the presence of chotts, the area is subjected to an important degree of vulnerability, encouraged by the discharges of wastewater (domestic and irrigation). These factors expose aquifer's water … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,108 accessesView PDFCite
Research ArticleOpen accessIn the keywordsWaste Management: A Case Study of Ongoing Traditional Practices at East Calcutta WetlandShaon Raychaudhuri, Madhusmita Mishra, Poulomi Nandy, Ashoke R. ThakurCalcutta has a unique waste management system which uses the traditional non conventional practice for treating both the solid as well as soluble waste for its 12 million inhabitants . It not only detoxifies the waste but also generates resources for the existing society in form of employment as well as edibles like sufficient vegetable, fish as well as paddy … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 8,058 accessesView PDFCite