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Research ArticleOpen accessEvery word in the titleCytotoxic Activity of Tinospora crispa Crude Extracts (Stem) Against K562 Human Leukemia CellsNormah Awang, Haziratul Iffah Ruzali, Rapidah Mohamad et al.Tinospora crispa (T. crispa) is traditionally used as an herbal medicine for treatment of gout and is an analgesic, anti-inflammatory and antihyperuricemic agent. Cytotoxicity of hexane, dichloromethane, ethanol and aqueous extracts of T. crispa stem against K562 cells was assessed. Crude extracts of T. crispa stem were obtained by sonication and maceration methods. T. crispa stem powder was macerated and … …OnLine Journal of Biological Sciences, Vol. 19, Issue 2 · 11 May 2019 · 7,870 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAutomatic Counting for Live and Dead Cells from Trypan Blue-Stained Images by Image Analysis Based on Adaptive K-Means ClusteringSu Mon Aung, Kanyanatt Kanokwiroon, Tonghathai Phairatana, Surapong ChatpunComputer-assisted image analysis can be employed to reduce the time consumed in the routine task such as cell counting. This study aimed to establish a method to perform this routine task based on an image analysis to automatically count live and dead cells after staining with trypan blue dye. Gray scale conversion and morphological operation were applied to the input … …Journal of Computer Science, Vol. 15, Issue 2 · 1 Mar 2019 · 8,566 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBulk and Interface Configuration on the Performances of Perovskite Solar CellsSankara Issiaka, Soumaïla Ouédraogo, Daouda Oubda et al.The ongoing search for energy conversion technologies that are more efficient, affordable, and sustainable than traditional solar cells have led researchers to look at other technologies. Among these technologies, perovskite-based solar cells seem increasingly credible, as they have the potential to achieve high energy conversion efficiencies that rival or even surpass those of silicon solar cells, which currently dominate the … …American Journal of Applied Sciences, Vol. 20, Issue 1 · 7 Nov 2023 · 6,018 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleProduction of Bioelectricity Using Single Chamber Microbial Fuel CellAhmad Nawaz, Ram Sharan SinghThe present study addressed the application of sandwich-type air cathode microbial fuel cell (ACMFC) for the production of bioelectricity. Activated carbon was pasted on aluminum mesh for the manufacture of anode and cathode. This electrode design eliminated the requirement for carbon cloth or a metal catalyst, resulting in a cathode with excellent activity for oxygen reduction. The findings demonstrated that … …American Journal of Environmental Sciences, Vol. 19, Issue 2 · 4 Sep 2023 · 5,735 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImmobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide DetectionChadaporn Pootawee, Witsanu Senbua, Jesdawan WichitwechkarnAs the result of the extensive use and high contamination of organophosphate (OP) insecticides in agricultural products and the environment, especially in developing countries, a simple and convenient means for OP detection and screening was investigated. Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 18 Sep 2018 · 7,673 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIsolation and Identification of Oral Cancer Stem Cells (CSC) within Commercially Available Cell LinesRobert Chauncey, Kai Hatch, Toni Jilka et al.Previous studies have demonstrated some tumors develop or maintain a small sub-population of cells with stem cell-like properties. This Cancer Stem Cells (CSC) may exhibit differential properties that allow their escape from traditional radiation or chemotherapy treatments and may therefore be responsible for cancer recurrence. Few studies have explored this phenomenon among oral cancer cell lines, therefore the objective of … …Current Research in Dentistry, Vol. 9, Issue 1 · 2 Jun 2018 · 12,719 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAnti-Proliferative Activity of Zerumbone Against Tumour Cell LinesAdel Sharaf Al-ZubairiZerumbone (ZER) is a cytotoxic phytochemical component extracted from the tropical herbal plant, Zingiber Zerumbet smith. It has been identified as an active anticancer compound. This study was aimed to evaluate in vitro the anti-proliferative activity of this bioactive compound, zerumbone, on seven cancer cell lines namely: CEMss (T4-lymphoplastoid), Chinese Hamster Ovary (CHO), 3T3 (mouse fibroblast), WEHI-3 (murine monomyelocytic leukemia), … …OnLine Journal of Biological Sciences, Vol. 18, Issue 2 · 25 Apr 2018 · 8,317 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRadiation Triggered Phenotypic Changes of Regulatory T CellsLantao Liu, Yan Peng, Deqin Zhang, Dongsheng NiuIonizing radiation disrupts the immune balance easily. Regulatory T (Treg) cells directly affect the immune balance after radiation exposure. Meanwhile, the present study is to investigate the survival ability and phenotypic changes of Treg cells induced by 60Co γ-rays in order to expand the understanding on how Treg cells modulated by radiation. Mice lymphocytes were isolated from thymus and spleen … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 20 Mar 2022 · 5,828 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleA New Approach to Model Concentrating PV Cells Under Non-Uniform IlluminationHang Zhou, Yuehong Su, Gang Pei et al.Concentrating PV (CPV) cells are often designed optimally depending on solar concentrator characteristics and operating conditions to ensure the best system performance. Before reaching a PV cell, solar radiation may experience some optical losses caused by the different physical phenomena occurring in a solar concentrator. A particular issue with the CPV technology is the non-uniformity of the concentrated radiation, which … …Energy Research Journal, Vol. 8, Issue 2 · 30 Aug 2017 · 7,137 accessesView PDFCite