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59 results for “Polymerase Chain Reaction (PCR)”
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Research ArticleOpen accessIn the abstractDetection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler BreedersAgustin Indrawati, Leila Nur Azizah, I Wayan Teguh Wibawan et al.The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 2 · 17 Jun 2020 · 7,010 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Commercial Fraud in Processed Meat Products Using Rapid TechniquesAli M. Ahmed, Takwa H. Ismail, Reham R. Abouelmaatti et al.Meat adulteration with different species has become a considerable problem worldwide. It means that meat products contain undeclared meat species; so, the meat ingredients are not consistent with the label. It has direct impact on public health, religious faith, fair-trades and food safety standers. This study focused on species adulteration detection in meat products by applying accurate, slandered and fast … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 7,821 accessesView PDFCite
Research ArticleOpen accessIn the abstractScreening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, IndonesiaSaleha Hannum, Hesti Wahyuningsih, Riyanto Sinaga et al.Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 25 May 2020 · 7,542 accessesView PDFCite
Research ArticleOpen accessIn the abstractVariations in Pathogenicity and Molecular Characterization of Infectious Bursal Disease Virus (IBDV) In EgyptMahmoud E. Sedeik, Ashraf M. Awad, Heba Rashed, Wael K. ElfeilInfectious bursal disease consider a vital threat to poultry industry worldwide and It has special potentiality in Egypt as the country become endemic with several clades from avian influenza from both types highly pathogenic (H5N1 and H5N8) and low pathogenic one (H9N2). The Egyptian government depends on the inactivated vaccine strategy to control avian influenza and any infection with IBD … …American Journal of Animal and Veterinary Sciences, Vol. 13, Issue 2 · 18 May 2018 · 8,485 accessesView PDFCite
Research ArticleOpen accessIn the abstractCariogenic Pathogen Scardovia Wiggsiae Screening Among Pediatric Orthodontic Patients: A Pilot StudyWeston Milne, Ghazaleh Rezaei, Adam Whiteley, Karl KingsleyDental caries remains one of the most prevalent oral health diseases in the United States, affecting nearly half of all children and a majority of adults. Most medically important cariogenic bacteria, including Streptococcus, Lactobacillus, Actinomyces and Veillonella species are well known, although recent evidence has identified the new cariogenic pathogen Scardovia wiggsiae (S. wiggsiae) among children and minorities with severe … …Current Research in Dentistry, Vol. 9, Issue 1 · 6 Oct 2017 · 11,875 accessesView PDFCite
Research ArticleOpen accessIn the abstractSex Identification and the Sex-Related Genes of Nipponia NipponFeilong Sun, Xuanfu Huang, Shuiyun YangSex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 2 · 30 May 2017 · 7,846 accessesView PDFCite
Research ArticleOpen accessIn the abstractImpact of Acute Bronchiolitis on Cardiac Functions and Serum microRNA-122 and 499Mona Abd El-Gelel Mohamed, Khalid Mahmoud ZayedBronchiolitis is an acute inflammatory injury of the bronchioles that is usually caused by a viral infection, most commonly Respiratory Syncytial Virus (RSV). This study aimed to verify the direct injurious effect of acute bronchiolitis on the myocardium and serum level of microRNA-122 and 499 (miR-122 and miR-499) of infected infants. Serum lactate dehydrogenase (LDH), creatine phosphokinase-isoenzyme MB (CK-MB) and … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 26 Jan 2016 · 7,259 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Association of HLA Class II Alleles with the Response to Alfa-Interferon/Ribavirin Therapy in Chronic Hepatitis C PatientsAwny Aly Abol-Ez Gawish, Nahla Abd El-Hamid Mohammed, Rasha Adel Sayed Ahmed Hussein, Elsaid Galal El-BadrawyHuman Leucocyte Antigens (HLA) class II appears to play an important role in the individual’s immune response to viral infection. The present study was aimed at assessment of the relationship between HLA DRB1 alleles and the response to HCV combined therapy in Chronic Hepatitis C patients (CHC). We enrolled a total of 44 chronically infected HCV patients without Hepatitis B … …American Journal of Immunology, Vol. 11, Issue 3 · 12 Nov 2015 · 6,704 accessesView PDFCite
Research ArticleOpen accessIn the abstractAmino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)Raksmont Ubonbal, Saijai Posoongnoen, Jureerut Daduang et al.The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 3 · 19 Aug 2015 · 7,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractOccurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MANathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves et al.The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 24 Jul 2015 · 8,946 accessesView PDFCite