Sex Identification and the Sex-Related Genes of Nipponia Nippon
Feilong Sun1, Xuanfu Huang2 & Shuiyun Yang2 Show affiliations
- Xi’an Polytechnic University, China
- Xi’an Jiaotong University, China
American Journal of Biochemistry and Biotechnology, Volume 13, Issue 2, pp. 81–84 · doi.org/10.3844/ajbbsp.2017.81.84
Abstract
Sex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp CHD gene fragments on Z chromosome in both sexes and a 463 bp female specific CHD fragments on W chromosome were cloned and sequenced. The sequences of these two sexing related genes fragments were similar with CHD-W of Phalacrocorax capillatuss and CHD-Z of Platalea minor individually. This study is valuable for managing and conserving endangered birds.
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Sun, F., Huang, X. & Yang, S. (2017). Sex Identification and the Sex-Related Genes of Nipponia Nippon. American Journal of Biochemistry and Biotechnology, 13(2), 81–84. https://doi.org/10.3844/ajbbsp.2017.81.84
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