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61 articles for “PCR detection”
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Research ArticleOpen accessIn the abstractDetection of Staphylococcus pseudintermedius in Dogs and Cats in Selangor, MalaysiaMohamed Abdelrahman Mohamed, Saleha Abd Aziz, Gurmeet Kaur Dhaliwal et al.Staphylococcus pseudintermedius is an emerging coagulase gram-positive staphylococci in dogs and cats. It has now become a threat to animal health due to its multidrug resistance leading to very limited therapeutic options particularly in the treatment of small animals and therefore, requires urgent action to control its spread. The organism is currently recognised as a significant pathogen in veterinary medicine … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 9 Jan 2020 · 7,968 accessesView PDFCite
Research ArticleOpen accessIn the abstractAlcohol Intubation and Synergistic Effect of Folic Acid and Egg Yolk Powder Solution on Alcohol Dehydrogenase [ADH] Class I Gene Expression and Single Nucleotide Polymorphism Frequency in Parent and Prenatal Alcohol Exposed Wistar RatsAdejoke Olukayode Obajuluwa, Oluyinka Ajibola Iyiola, Joseph Akintade Morakinyo et al.Point mutation detections in alcohol metabolizing genes could unravel molecular targets of gestational alcohol and epigenetic remodeling capacity of maternal nutrition, which underscores growth and health endpoints in Pre­natal Alcohol Exposed (PAE) fetuses. gene expression was assayed using sequenced target and internal control gene primers in a two-step thermal cycling process from isolated RNA in purely bred alcohol male and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 22 Nov 2023 · 4,859 accessesView PDFCite
Research ArticleOpen accessIn the abstractTetracycline Resistance Profile in Darwin Finches in the Galapagos IslandsMaría Inés Baquero, Marylin Cruz, Viviana Duque et al.Antimicrobial Resistance (AMR), which is the ability of microorganisms to withstand attack by antimicrobial drugs, has a worldwide impact. Thus, it is important to identify resistance mechanisms circulating in the environment. Among antimicrobials widely used in human and veterinary health, tetracycline is ideal due to its safety and has been categorized by the WHO as a critically important antimicrobial. Based … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 4 · 19 Dec 2022 · 5,794 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Detection of Follicle Stimulating Hormone Receptor Polymorphisms and Luteinizing Hormone Gene Variations in Primary and Secondary Iraqi Infertile WomenNoor Basim Naser, Dr. Ezeddin Atia Albayyar, Dr. Susan Abed ZaidanThe current study aimed to determine the role of hormonal disturbances in infertility, moreover assessing variations at the molecular level that could affect this disease. The study examined 90 blood samples, 30 patients with primary infertility, 30 patients with secondary infertility and 30 healthy women as control. FSH and LH were measured using Electrochemiluminescence assay. DNA was isolated for molecular … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 15 Nov 2018 · 7,558 accessesView PDFCite
Research ArticleOpen accessIn the abstractSensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow DipstickZezhen Li, Yun Xiong, Peng Zhu, Hailong HuangThe existence of Sulfate-Reducing Bacteria (SRB) is one of the significant reasons for the Microbially Influenced Corrosion (MIC) of jet fuel. Especially for the jet fuel stored by military, since jet fuel is stored in the tank for a long time, some oxygen-consuming bacteria such as Amorphotheca resinae and Bacillus Cohn can consume oxygen and generate organic acids at the … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 11 Apr 2018 · 10,398 accessesView PDFCite
Research ArticleOpen accessIn the abstractMultidrug-Resistant Bacterial Pathogens Assessment in Canine Ophthalmic InfectionsRicardo Antonio Pilegi Sfaciotte, Lincoln Garcia Coronel, Alessandra Snak et al.The objective of this study was to identify the main microorganisms associated with ophthalmic infections and determine the resistance profile of these isolates against antimicrobial drugs. 26 bacterial isolates from 18 canine ophthalmic infections were submited to the phenotypic resistance profile for 36 drugs of 12 classes of antimicrobials, research of multidrug-resistant strains with importance in public health and detection … …American Journal of Animal and Veterinary Sciences, Vol. 13, Issue 1 · 2 Jan 2018 · 7,280 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of HSV1 and HSV2 among HIV Patients in Northern South Africa as Determined by Real Time PCR in Urine SamplesAmidou Samie, Hlayisani Preclude Mnisi, Mary Noluthando RamantswanaThe present study determined the prevalence of HSV-1 and 2 among HIV patients in Northern South Africa and identified potential risk factors. Urine samples were collected from patients attending different HIV clinics in Limpopo Province. Total genomic DNA was isolated from these samples using GenElute Blood Genomic DNA Kit and real time PCR protocols were used for the detection of … …American Journal of Infectious Diseases, Vol. 13, Issue 1 · 18 Jul 2017 · 7,915 accessesView PDFCite
Research ArticleOpen accessIn the abstractWhat is Missing in STD Screening in Hong Kong?Clement Leung-Kwok Chan, Wai Yee Chan, Christopher Hon Ki Cheng, Ping XiaThis retrospective analysis was to study the prevalence of Sexually Transmitted Diseases (STD) in a Reproductive Medical Center in Hong Kong. A total of 1190 patients were included in this study. Group 1A, couples had no symptoms but presented with subinfertility; Group 1B, the subfertile couples were positive for either Chlamydia trachomatis (CT) or Ureaplasma urealyticum (UU); Group 2, couples … …Current Research in Medicine, Vol. 7, Issue 2 · 11 Jan 2017 · 8,409 accessesView PDFCite
Research ArticleOpen accessIn the abstractEpidemiological Study on the Current Status of Rice Tungro Disease in South Sulawesi, IndonesiaNur Rosida, Tutik Kuswinanti, Nur Amin, Andi NasruddinIntegrated management of Rice Tungro Disease (RTD) by combining the appropriate planting time and cultivar rotation has successfully controlled the disease in South Sulawesi, Indonesia. By the early 1990’s, about 10 years after the management was implemented, tungro disease incidence was so low that the integrated management was no longer strictly implemented and eventually abandoned. Surprisingly however, the tungro incidence … …OnLine Journal of Biological Sciences, Vol. 20, Issue 4 · 3 Dec 2020 · 7,059 accessesView PDFCite
Research ArticleOpen accessIn the abstractFrequency of Single Nucleotide Polymorphisms of the SLCO1B1 Gene in Slavic Population of Central EuropeMichaela Mikulová, Veronika Kramarova, Ján ChandogaThe organic anion transporting polypeptide 1B1 (encoded by SLCO1B1 gene) is involved in the active cellular influx of diverse endogenous compounds and several drugs, such as HMG-CoA reductase inhibitors (statins). Two common polymorphisms c.388A>G and c.521T>C in SLCO1B1 alter transport activity of this transporter and play an important role in the pharmaceutical response to many drugs. The aim of our … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 11 Dec 2016 · 7,313 accessesView PDFCite