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67 articles for “In vitro Culture”
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Research ArticleOpen accessIn the abstractEvaluation of Antioxidant and Antibacterial Activities of the Polysaccharides from Mycelium and the Culture Medium of Pleurotus Eryngii Strain UN-1Qing Liu, Haodong Wu, Ying Wu et al.Pleurotus eryngii was one of the most precious and ordinary edible mushrooms, and its polysaccharides were the major active substances. In this study, P. eryngii strain UN-1 was cultured in a liquid medium at 25℃ under a shaking of 80 rpm for 7 days. Two crude polysaccharides named CPN and CPNM were obtained from the mycelium of P. eryngii UN-1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 19 Jul 2022 · 6,176 accessesView PDFCite
Research ArticleOpen accessIn the abstractInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Rumen Fermentability of the Pelleted Feed Containing Water Spinach (Ipomoea aquatica)Himmatul Hasanah, Eko Pangestu, Ali Agus, Joelal AchmadiAn in vitro technique based on two stages of batch culture was used to clarify fermentability and digestibility of pellet feed containing water spinach (Ipomoea aquatica). The portion of Pennisetum purpureum in the pellet feed was substituted with Ipomoea aquatica at respective levels of 0% (T1), 20% (T2), 40% (T3) and 60% (T4). The treatments were allocated in a completely … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 27 Mar 2020 · 7,559 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Rumen Culture Adaptation to Origanum vulgare L. Essential Oil on Rumen Methane and FermentationMohamed G. Embaby, Mevlüt Günal, Amer AbuGhazalehThe main objective of this study was to investigate the impact of adding oregano essential oil (OEO) to adapted and unadapted rumen cultures on methane gas (CH4) production and rumen fermentation under in vitro condition. Rumen fluid for culture fermenters was collected from a Holstein cow located in Southern Illinois University farms. The adapted and unadapted rumen cultures were obtained … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 3 · 2 Sep 2019 · 7,599 accessesView PDFCite
Research ArticleOpen accessIn the abstractFlavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro PlantletsEvgeniya A. Karpova, Alexandra Yu. Nabieva, Tatiana D. Fershalova et al.The objective of this study was to design a protocol of the successful establishment of plants of Begonia fischeri var. palustris obtained in the in vitro seeds culture, to evaluate flavonoid content and antimicrobial properties of in vitro plantlets. The significant increase of percentage of seed germination (92.5%) was recorded in the half strength MS medium and 1% agar medium … …OnLine Journal of Biological Sciences, Vol. 19, Issue 1 · 29 Jan 2019 · 7,649 accessesView PDFCite
Review ArticleOpen accessIn the abstractSpermatogonial Stem Cells: A Current Update in the Management of Azoospermic Infertile MenSilvia W Lestari, Debby AdityaOne of the management of azoospermia is utilizing Assisted Reproductive Technologies (ART) by Intra-Cytoplasmic Sperm Injection (ICSI) with sperm obtained from the epididymis or directly from testis. In some conditions such as hypogonadism or undescended testis, the sperm are not successfully obtained. Thus, it is considered to perform Spermatogonial Stem Cells (SSCs) culture to restore men fertility. The objective of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 5 Dec 2017 · 6,923 accessesView PDFCite
Research ArticleOpen accessIn the abstractModeling Hepatocellular Toxicity of Enavatuzumab, a Humanized Anti-TweakR AntibodyDonghee Choi, Yanhong Zhu, Debra Tien Chao et al.In a Phase 1 clinical study, treatment with enavatuzumab, a humanized monoclonal antibody to TweakR, resulted in liver toxicity in a subset of patients. The objective of this current study was to evaluate the ability of preclinical studies to predict liver toxicity in humans. Enavatuzumab was evaluated in cynomolgus monkeys, where serum liver enzyme and cytokine levels were measured and … …American Journal of Pharmacology and Toxicology, Vol. 12, Issue 2 · 9 Nov 2017 · 7,719 accessesView PDFCite
Research ArticleOpen accessIn the abstractMetabolite Profiles of Arsenic Tolerant Plants Regenerated from Stem Calli of Andrographis paniculata (Burm.f.) Nees using HPLC and 1D 1H NMRJ. Vijayakumar, P. Ponmanickam, P. Samuel et al.In vitro culture provide a suitable condition for regeneration of arsenic tolerant plants from stem calli of Andrographis paniculata (Burm.f.) Nees. The regenerated plants could be valuable material applicable to soil remediation. In vitro culture of stem explants on MS basal salts, 3.0% sucrose, 0.8% agar medium fortified with various concentrations of As2O3 (0.0-9.0 µM) along with NAA (3.5 mg … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 27 Oct 2017 · 7,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of in vitro Rooting through Growth Media, Gelling Agents and Activated Charcoal in Lycium chinenseJae Kwang Kim, Sang Un ParkLycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 26 Jun 2017 · 7,267 accessesView PDFCite
EditorialOpen accessIn the abstractIs Rerum® the New Coley's Vaccine?Marco RuggieroColey's Vaccine and Cancer Immunotherapy Dr. William B. Coley is credited for having been the first to translate the observation of an association between bacterial infections, high fever and cancer remission into a successful cancer treatment. However, it is worth noting that such an association dates back to at least one century before Coley's times; as Wiemann and Starnes from … …American Journal of Immunology, Vol. 13, Issue 2 · 31 May 2017 · 12,281 accessesView PDFCite