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39 results for “Clone”
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Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Review ArticleOpen accessIn the abstractThe Triple Immune Argument; Surveillance/Evasion/ Senescence and the Increased Incidence of Acute Myeloid Leukemia Observed with AgeManar M. IsmailAML originates from genetic insults of hematopoietic stem and progenitor cells (HSPCs/HSCs) that was identified earlier as leukemia stem cells (LSCs). In quiescent state, trafficking immune cells are crucial for eradication of aberrant clones and obtaining balance between proliferation and apoptosis to maintain HSCs pool. Regulatory T-cells shield the HSCs from the inflammatory reactions by suppressing T- and B- cells. … …American Journal of Immunology, Vol. 13, Issue 4 · 22 Dec 2017 · 7,806 accessesView PDFCite
Research ArticleOpen accessIn the abstractMetabolite Profiles of Arsenic Tolerant Plants Regenerated from Stem Calli of Andrographis paniculata (Burm.f.) Nees using HPLC and 1D 1H NMRJ. Vijayakumar, P. Ponmanickam, P. Samuel et al.In vitro culture provide a suitable condition for regeneration of arsenic tolerant plants from stem calli of Andrographis paniculata (Burm.f.) Nees. The regenerated plants could be valuable material applicable to soil remediation. In vitro culture of stem explants on MS basal salts, 3.0% sucrose, 0.8% agar medium fortified with various concentrations of As2O3 (0.0-9.0 µM) along with NAA (3.5 mg … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 27 Oct 2017 · 7,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractSex Identification and the Sex-Related Genes of Nipponia NipponFeilong Sun, Xuanfu Huang, Shuiyun YangSex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 2 · 30 May 2017 · 7,841 accessesView PDFCite
Research ArticleOpen accessIn the abstractA NEW PRESUMED IGKAPPA GENE IMPLIED IN THE SEA STAR IMMUNE RESPONSEMichel Leclerc, Magne Osteras, Patricia OttenThe existence of another candidate IgKappa gene of 697 nucleotides, was recently, demonstrated, in the sea star Asterias rubens genome. This last gene was cloned and shown, with its sequence, in the present study. Its potential role in the sea star immune response was accentuated. …American Journal of Immunology, Vol. 10, Issue 1 · 19 Mar 2014 · 6,788 accessesView PDFCite
Research ArticleOpen accessIn the abstractA SHORT INTERFERING RNA MOLECULAR BEACON FOR THE ATTENUATION OF MYCOBACTERIAL INFECTIONRemo George, Kathy Nugent, Norman Bolus et al.The ability of the pathogen Mycobacterium Tuberculosis (MTB) to invade and survive within macrophages of granulomas is attributed to the product of the Mammalian Cell Entry (MCE) operon whose gene, mce4A, encodes a cholesterol transporter that transports host lipids into the bacterium that allows the bacterium to survive during chronic infection. Here, we proposed and tested the hypothesis that a … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 4 Mar 2014 · 7,774 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessIn the abstractHigh-Yield Production of Bioactive Flatfish (Paralichthys olivaceus) Myostatin-1 Prodomain in Escherichia coliIl Shik Shin, Hyung-Joo Jin, Jeong Hwan Kim et al.Myostatin Propeptide (MstnPro), the N-terminal part of Mstn precursor molecule, binds to Mstn and suppress its activity. Mstn is a potent negative regulator of skeletal muscle growth in many animal species, thus MstnPro has a great potential to be used as an agent to enhance skeletal muscle growth in animal species. Bioactive fish Myostatin-1 Propeptide (Mstn1Pro) proteins were recently expressed … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 3 · 20 Sep 2012 · 8,012 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment of Transgenic Maize using Immature Embryos of HiII Genotype as a Vaccine CandidateHyun A. Kim, Suk Yoon Kwon, Han Sang Yoo et al.Problem statement: Plant-based vaccines posses some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs and wider use of vaccines for veterinary medicine. These study was undertaken to develop the transgenic maize as edible vaccine candidate for animals. Approach: The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 1 · 5 Mar 2012 · 7,567 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiscovery of Evolutionary Divergence of Biological Nitrogen Fixation and Photosynthesis: Fine Tuning of Biogenesis of the NifH and the ChlL by a Peptidyl-Prolyl Cis/Trans IsomeraseNara Gavini, Sinny Delacroix, Kelvin Harris Jr., Lakshmi PulakatProblem statement: Despite the structural and functional similarities between the nitrogenase that performs biological nitrogen fixation reaction and the Dark Protochlorphyllide Oxidoreductase (DPOR) that performs chlorophyll-biosynthesis, attempts to substitute nitrogenase-components with DPOR-components have hitherto failed. This investigation was undertaken to test if Chlamydomonas reinhardtii protochlorophyllide (Pchlide) reductase (ChlL) that shares some structural similarity with Nitrogenase Reductase (NifH) could complement the … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 17 Feb 2012 · 7,614 accessesView PDFCite