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68 articles for “in vitro Analysis”
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Research ArticleOpen accessIn the abstractComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDFCite
Research ArticleOpen accessIn the abstractEfficiency of Nitrogen Fixation by Rhizobium Strains Associated With White Clover in the Ecuadorian Highlands Biological Nitrogen Fixation by White Clover in EcuadorDaniela Michell Chuquimarca Mamarandi, Venancio Saúl Arahana Bonilla, Paulina Andrea Cevallos Hidalgo et al.The objective of this research was to evaluate the nitrogen-fixing capacity of 30 strains of Rhizobium isolated from white clover nodules, collected in the provinces of Pichincha and Cotopaxi under greenhouse conditions. After isolation of the bacteria and authentication of the isolates, a greenhouse experiment was conducted, in a Completely Randomized Design (CRD), with three replications and two controls: with … …OnLine Journal of Biological Sciences, Vol. 26, Issue 4 · 4 Oct 2026 · 835 accessesView PDFCite
Research ArticleOpen accessIn the abstractQualitative Analysis of the Chemical Composition of Cinnamomum tamala leaves and Biological ActivityQianwen Gong, Pin Chen, Linyang Wang, Shuge TianCinnamomum tamala (Buch.-Ham.) Nees and Ebermaier. (Lauraceae) is a less explored medicinal plant in Uyghur medicine, with limited knowledge about its non-volatile compounds. We utilized chemical pre-screening in conjunction with UPLC-Q-Orbitrap-MS to analyze the phytochemical makeup of C. tamala leaves. Total polyphenols were measured using the Folin-Ciocalteu method, while total flavonoids were quantified through NaNO2-Al (NO3)3 colorimetric analysis across four … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 21 Sep 2026 · 659 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the abstractAqueous Extracts of Bay Leaf (Laurus nobilis) and Rosemary (Rosmarinus officinalis) Inhibit Iron-Induced Lipid Peroxidation and Key-Enzymes Implicated in Alzheimer’s Disease in Rat Brain-in VitroAyodeji O. Falade, Goodness I. Omolaiye, Kayode E. Adewole et al.Neurological disorders remain a global health challenge, with Alzheimer’s Disease (AD) as the most famous cause of dementia in old people. Nevertheless, culinary spices and herbs have shown promising potentials in the management of neurological diseases. This study aimed at investigating the neuroprotective property of aqueous extracts of bay leaf (Laurus nobilis) and rosemary (Rosmarinus officinalis) by assessing the antioxidant … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 25 Jan 2022 · 7,955 accessesView PDFCite
Research ArticleOpen accessIn the abstractSupplementation of Prill Fat Derived from Palm Oil on Nutrient Digestibility and Dairy Cow PerformanceLolita Udin Riestanti, Despal, Yuli RetnaniPrill fat is a hydrogenated vegetable oil which contains more than 85% palmitic acid with a high melting point. Due to this reason, it does not melt at rumen temperature and bypass rumen degradation. This study was aimed to determine the optimum level of prill fat (86% palmitic acid) supplementation in dairy ration on fermentation characteristics, feed digestibility, milk production, … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 2 · 1 Jul 2021 · 8,750 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of the Protein Profile of Cassava Plantlets (Manihot esculenta Crantz.) Resistance to Fusarium Wilt DiseaseEndang Nurcahyani, Hardoko Insan Qudus, Ferina EvlinFusarium wilt disease is still a production constraint in Cassava (Manihot esculenta Crantz.) cultivation. The disease is caused by the fungus Fusarium oxysporum (Fo), which to date has not been cured. It is expected that the use of varieties of cassava that are resistant to Fusarium wilt is an important alternative for disease control. Induced Resistance cassava research on Murashige … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 18 Jun 2021 · 6,777 accessesView PDFCite
Research ArticleOpen accessIn the abstractSuppression of Bipolaris Leaf Blotch and Improvement of Wheat Growth by Plant Growth Promoting Rhizobacteria BacilliFarzana Zerin Eivy, Md. Tanbir Rubayet, Umakanta Sarkar, Md. Motaher HossainBipolaris leaf blotch, induced by Bipolaris sorokiniana, poses a notable risk to wheat cultivation, resulting in considerable annual declines in yield. Plant Growth-Promoting Rhizobacteria (PGPR) are acknowledged for their colonization of the wheat rhizosphere and provision of various advantages to plants, including disease control and improved growth. This investigation seeks to explore the feasibility of utilizing PGPR strains as a … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 12 Mar 2024 · 6,637 accessesView PDFCite
Research ArticleOpen accessIn the abstractMechanism Analysis of Artemisinin and Artesunate as Lung Cancer Therapy Agents Through in Silico MethodDidik Harmoko, Vivitri Dewi Prasasty, Adi YulandiSeveral in vitro studies have focused on the ability of artemisinin and its derivatives to suppress lung cancer cells, but there are still many biological processes and mechanisms, such as the interactions between the active compounds (ligands) and receptors that are not well understood and require further research. Drug targeting for lung cancer will be more precise and the inhibition … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 15 Feb 2024 · 6,144 accessesView PDFCite
Research ArticleOpen accessIn the abstractCytotoxicity Evaluation of Curcumin-FITC Conjugates in Granulosa Cells Isolated from Ovarian Follicles of Kampung Chicken (Gallus gallus domesticus)Sukarman Hadi Jaya Putra, Mansur S, UmarudinLinking curcumin to fluorescein isothiocyanate (FITC) results in a hybrid fluorescent marker (curcumin-FITC) that exhibits improved stability and sensitivity in detection. This study sought to evaluate the biological safety and cytotoxic effects of curcumin-FITC on granulosa cells obtained from ovarian follicles (2-5 mm, F1-F4 stage) of Kampung chickens. Following fluorescent labeling with different concentrations of curcumin-FITC (0-100 μg/mL), cell viability … …OnLine Journal of Biological Sciences, Vol. 26, Issue 2 · 14 May 2026 · 1,497 accessesView PDFCite