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36 articles for “hydrophobic”
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Research ArticleOpen accessIn the keywordsPapain, a Plant Enzyme of Biological Importance: A ReviewFlorence Mamboya, Ezekiel AmriPapain is a plant proteolytic enzyme for the cysteine proteinase family cysteine protease enzyme in which enormous progress has been made to understand its functions. Papain is found naturally in papaya (Carica papaya L.) manufactured from the latex of raw papaya fruits. The enzyme is able to break down organic molecules made of amino acids, known as polypeptides and thus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 44,698 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Methodology for the Selection of Micro-Assembly TechniquesDario Antonelli, Gualtiero Fantoni, Marcello Porta, Marco SantochiProblem statement: The study addressed the problem of selecting the appropriate microassembly techniques according to the micro-part features. Actually, in the micro-domain, the choice of the correct assembly technique is highly dependent on the micro-part characteristics such as shape, geometry and material. Approach: Since there was an incomplete and unstructured knowledge about the micro-assembly, the study proposed a Decision Support … …American Journal of Engineering and Applied Sciences, Vol. 3, Issue 4 · 5 Nov 2010 · 8,176 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn silico and in vitro Structural Analysis on the Interaction of Calmodulin and Calmodulin-Binding Motif of FKBP35 from Plasmodium knowlesiSazlinawatie Aladin, Cahyo Budiman et al.FK506-binding protein 35 (FKBP35) is a peptidyl-prolyl cis-trans isomerase found in Plasmodium knowlesi, a zoonotic malaria parasite responsible for concerning cases of malaria infection in East Malaysia. This protein contains a segment known as the calmodulin-binding motif (Pk-CBM), which is predicted to facilitate interaction with calmodulin from P. knowlesi (Pk-CaM). Although this interaction is considered promising for future antimalarial drug … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 10 Mar 2025 · 4,846 accessesView PDFCite
Research ArticleOpen accessIn the abstractInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,569 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of Geobacillus thermodenitrificans Lipase Activity and Binding Affinity by Acetone Treatment: Kinetic and in silico InvestigationsAmmar Khazaal Kadhim Almansoori, Alyaa Abdulhussein Alsaedi et al.Lipase Geobacillus thermodenitrificans (LipGt) is a thermostable enzyme that has an optimal activity temperature of 65°C. It can tolerate various organic solvents and has an improved activity, especially in the presence of acetone. In this study, in-vitro and in-silico studies were carried out to investigate the effect of acetone in enhancing the kinetic and binding affinity of LipGt. The LipGt … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 10 Oct 2024 · 4,817 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Trilobatin on Bovine Serum Albumin: Interaction Mechanism and Molecular DockingYuhan Zhai, Yuqing Zhang, Yaping Li et al.Understanding the transport and distribution of small molecules in vivo is aided by studying their interactions with proteins. As a natural sweetener, trilobatin possesses several biological activities. However, uncertainty surrounds the trilobatin and Bovine Serum Albumin (BSA) interaction mechanism. In this study, the binding types, number of binding sites and binding constants of trilobatin interaction with BSA were studied by … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 29 Mar 2024 · 4,843 accessesView PDFCite
Research ArticleOpen accessIn the abstractcDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2Joshua Evans M. Bajao, John Donnie A. RamosSuidasia pontifica (Sp) is a House Dust Mite (HDM) species found in tropical urban residential areas which causes sensitization of up to 75% of allergic patients. Cloning of allergens from Sp is a significant initial step in understanding the clinical significance of this HDM species. The study describes the cloning and in silico characterization of the group 2 allergen gene … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 14 May 2020 · 7,885 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coliDessy Natalia, Christian Heryakusuma, Fernita Puspasari et al.Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 10 Oct 2018 · 10,346 accessesView PDFCite
Research ArticleOpen accessIn the abstractAdsorption Behavior of Bone Morphogenetic Protein-2 on a Graphite Substrate for Biomedical ApplicationsIzabele Marquetti, Salil DesaiThe interaction of proteins with biomaterials plays an important role for several biomedical applications including implants and tissue engineering. Although different experimental approaches have been explored, the investigation of the molecular models for protein-substrate interaction is warranted to control of cellular functions. In this study, Molecular Dynamics (MD) simulations were used to study the adsorption behavior of Bone Morphogenetic Protein-2 … …American Journal of Engineering and Applied Sciences, Vol. 11, Issue 2 · 19 Jun 2018 · 8,705 accessesView PDFCite
EditorialOpen accessIn the abstractIs Rerum® the New Coley's Vaccine?Marco RuggieroColey's Vaccine and Cancer Immunotherapy Dr. William B. Coley is credited for having been the first to translate the observation of an association between bacterial infections, high fever and cancer remission into a successful cancer treatment. However, it is worth noting that such an association dates back to at least one century before Coley's times; as Wiemann and Starnes from … …American Journal of Immunology, Vol. 13, Issue 2 · 31 May 2017 · 12,281 accessesView PDFCite