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Research ArticleOpen accessIn the abstractIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Genetic Potential of Spring Durum Wheat Grain Quality in the North of KazakhstanS. M. Dashkevich, M. U. Utebayev, O. O. Kradetskaya et al.The paper presents information about the cultivation of spring durum wheat in the conditions of Northern Kazakhstan. Methods for assessing the quality of grain and semolina in the breeding process are shown: SDS sedimentation, protein content according to Kjeldahl, content, and index of gluten using the Glutomatic 2200 system, vitreousness, hardness SKCS 4100, content of carotenoid pigments, measurements of the … …OnLine Journal of Biological Sciences, Vol. 22, Issue 3 · 2 Sep 2022 · 6,087 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of Collagen from Parrotfish (Chlorurus Sordidus), Tiger Grouper (Epinephelus Fuscoguttatus) and Pink Ear Emperor (Lethrinus Lentjan): Effect of Acetic Acid Concentration and Extraction TimeAsep Awaludin Prihanto, Abdul Aziz Jaziri, Mohammad D. Pratomo et al.The use of marine fish's skin waste such as parrotfish (Chlorurus sordidus), tiger grouper (Epinephelus fuscoguttatus), and pink ear emperor (Lethrinus lentjan) needs improvement to increase its economic value and sustainability. Therefore, this study aims to investigate the effect of fish skin to acetic acid ratio with different extraction times on the yield and characteristics of skin collagen obtained from … …OnLine Journal of Biological Sciences, Vol. 22, Issue 1 · 28 Jan 2022 · 6,675 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Ferrous Ion Chelating Properties of Collagen Peptides from Dried and Fresh Cod SkinMengqian Chen, Cen Chen, Junchao Wu et al.Fish skin is a potential source of collagen and collagen is a protein source of bioactive peptides including iron chelating peptides. The aim of this study is to compare physicochemical properties of collagen extracted from fresh and dried cod skin and the ferrous ion chelating properties of collagen hydrolysates. Then the fresh and dried cod skin extracted collagen was hydrolyzed … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 3 · 2 Jul 2021 · 7,155 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 CellsShuqing Yang, Zhike Xie, Shuyan Yu et al.In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 26 Apr 2020 · 7,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Venom-Specific Fingerprints of Clinically Relevant Uruguayan Snakes by MALDI-TOFNorma Suarez, Victor MoraisSnakebite envenomation is a particular public health problem in many areas of the world, including extensive areas of Latin America. In particular, in Uruguay, snakebite accidents are caused by two snakes of the genus Bothrops: Bothrops alternatus and Bothrops pubescens. The only effective treatment available is the administration of the specific antivenom. Although there is an ELISA that allows the … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 26 Jul 2019 · 8,205 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Research ArticleOpen accessIn the abstractElectrophoretic Identification of Casein in Various Types of MilkMakhpal Yelubaevna Yelubaeva, Shynar Yırmkızy Kenenbay, Mirash Hudretovna Narmuratova et al.Casein proteins of camel milk were obtained from skimmed milk by sedimentation (pH 4.6) at 24°C. To sediment proteins from mare milk, pH was increased to 4.6 and milk was centrifuged at 45,000 rpm for 30 minutes at 4°C. The supernatant obtained after centrifugation was heated up to 30°C for further fractionation of whey proteins. The sedimented casein from camel … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 1 Dec 2017 · 8,134 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractExperimental Evaluation of the Raking Energy in Damping System of Steel Stud Partition WallsSalah Amer, Sameer Hamoush, Taher M. Abu-LebdehNonstructural partition walls are usually specified based on the best practice without any structural design calculations. However, given the likely severe earthquake damage to nonstructural components and the substantial cost of repair to such components, it is imperative that we develop an understanding of the seismic response of the nonstructural partition walls when subjected to earthquake loading conditions. The main … …American Journal of Engineering and Applied Sciences, Vol. 8, Issue 4 · 6 Nov 2015 · 7,640 accessesView PDFCite