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291 articles for “PCR”
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Research ArticleOpen accessEvery word in the titleDetection and Localization of Anti and Pro-apoptotic mRNA Genes in Human Colorectal Cancer Using in situ RT-PCRMohd. Nazil Salleh, Boo Siaw Shi, Wan Khairuzzaman Wan Ramli et al.Problem statement: Recent studies using conventional Polymerase Chain Reaction have shown that anti-apoptotic (Cyclooxygenase-2, COX-2 and Nuclear Factor-κB, NF-κB) and proapoptotic mRNA (Bax and Bad) are involved in the tumorigenesis of colorectal cancer. The aim of this study was to localize the expression of anti and pro-apoptotic mRNA genes using Reverse Transcription in situ Polymerase Chain Reaction (RT in situ … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,441 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImproving a PCR-Based Method for Identification of Ralstonia solanacearum in Natural Sources of West MalaysiaReza Khakvar, Kamaruzaman Sijam, Wong Mui Yun et al.Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 9,469 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssessment of Genotoxic Activity of Para-nitrophenol in Higher Plant Using Arbitrarily Primed- polymerase Chain Reaction (AP-PCR)Mohamed R. EnanParanitrophenol is a common toxic environmental pollutant; the aim of this study was to evaluate the potential of the DNA fingerprinting by AP-PCR assay to detect the DNA damage in the common bean (Phaseolus vulgaris) exposed to different concentrations of paranitrophenol (4.0-720 nM). The changes occurring in the fingerprint patterns were likely to be the result of paranitrophenol-induced DNA damage. … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 2 · 30 Jun 2007 · 7,064 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRandom Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman et al.Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 8,606 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleTrends in the Prevalence of Clostridioides difficile Using Real Time PCR in South Korea (2020-2024)Yonghee Lee, Yoo Na Chung, Jae Kyung KimClostridioides difficile is a major cause of infection in healthcare settings, and frequent antibiotic use exacerbates its occurrence. C. difficile infection (CDI) is a major public-health challenge owing to its complications and high mortality rate. We aimed to analyze current trends in C. difficile prevalence. This study was a retrospective, single center analysis of 7,371 samples from April 2020 to … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 3 Oct 2025 · 2,358 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImpact of Multiplex PCR Panel on Reducing Broad-Spectrum Antibiotic Use in Ventilator-Associated PneumoniaMaría Inés Staneloni, Emilio F. Huaier Arriazu et al.The extended use of broad-spectrum antimicrobials contribute to the growing threat of antimicrobial resistance. This study evaluated the impact of a multimodal strategy aiming at reducing the duration of broad-spectrum antimicrobial therapy in Ventilator-Associated Pneumonia (VAP). Conducted in a single Intensive Care Unit (ICU), this quasi-experimental, retrospective study compares a pre-intervention period (08/01/2018-07/01/2019) with an intervention period (08/01/2022-07/01/2023). Adult patients … …American Journal of Infectious Diseases, Vol. 21, Issue 1 · 8 Jul 2025 · 6,582 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePCR Diagnosis for the Identification of the Virulent Gene of Salmonella in Poultry MeatD. K. Zhanabayeva, A. Y. Paritova, G. K. Murzakaeva et al.The purpose of this study was to conduct microbiological studies of poultry meat imported into Mongolia and establish the serotypes and plasmids of Salmonella and determine the virulence of the gene encoding antibiotic resistance and its transmigratory properties. The PCR molecular biological method was used and according to the results, Salmonella enteritidis was identified in chicken carcasses of the American … …OnLine Journal of Biological Sciences, Vol. 21, Issue 3 · 27 Aug 2021 · 6,865 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePrevalence of HSV1 and HSV2 among HIV Patients in Northern South Africa as Determined by Real Time PCR in Urine SamplesAmidou Samie, Hlayisani Preclude Mnisi, Mary Noluthando RamantswanaThe present study determined the prevalence of HSV-1 and 2 among HIV patients in Northern South Africa and identified potential risk factors. Urine samples were collected from patients attending different HIV clinics in Limpopo Province. Total genomic DNA was isolated from these samples using GenElute Blood Genomic DNA Kit and real time PCR protocols were used for the detection of … …American Journal of Infectious Diseases, Vol. 13, Issue 1 · 18 Jul 2017 · 7,911 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUse of Enterobacterial Repetitive Intergenic Consensus PCR in Detecting Target(s) of Hapalindole-T, From a Cyanobacterium, in Escherichia Coli: In Silico ValidationManoj K. Tripathi, Maheep Kumar, Vinay K. Singh et al.Problem statement: Identification of newer biomolecule as well as targets are a rising concern because of increasing drug resistance in bacteria. We have isolated a broad spectrum antibiotic biomolecule Hapalindole-T from a cyanobacterium, Fisherella sp. growing on local Azadirachta indica tree bark. A model bacterium E. coli was screened spontaneously for Hap-T resistance. These resistant strains of E. coli were … …Current Research in Bioinformatics, Vol. 1, Issue 1 · 3 Mar 2012 · 13,777 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar WashElizangela Maira Dos Santos, Pedro Moreira Couto Motta, Marcos Bryan Heinemann et al.Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectious disease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experiment was compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) and Bronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samples isolated from PBMCs and BAW of naturally infected animals were … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 7,462 accessesView PDFCite