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291 results for “PCR”
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Research ArticleOpen accessEvery word in the titleEffects of Aerobic Exercise on PPAR-γ Expression in Adipose Tissue and RT-PCR Detection AnalysisXiaodong Zhan, Chenguang Bi, Jie WeiThis study aimed to examine the effect of organized aerobic exercise on the expression level of Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) gene in adipose tissue by performing digital variant of RT-PCR, Droplet Digital PCR (ddPCR) which is a highly sensitive method for counting the gene copy numbers. The proposed scheme consists of three-stage analytical procedure, namely, Subphase I Controlled Aerobic … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 29 Sep 2026 · 580 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImpact of Integrated Nursing and Digital Droplet PCR on Bullous Dermatoid Conditions: A Randomized Controlled TrialConghui ChenBullous skin disease is an autoimmune disease characterized by the formation of blisters on the skin or mucous membrane, which seriously impairs the quality of life and overall health. Similar to the research on biomolecules (mechanics of protein interaction), cells (including cell membrane elasticity, cell adhesion, cytoskeletal dynamics, response to mechanical stimulation, cell deformation and cell endoforce transfer) and tissue … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 2 · 2 Jul 2026 · 985 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCpG Oligodeoxynucleotides Debate the Damaging Action of Cisplatin on Immune Cell DNA: A PCR Array Application on Repair Genes ExpressionNaglaa Fathy Alhusseini, Amal Abo El-Fadl, Shabaan Hemada et al.Synthetic Oligodeoxynucleotides (ODN) expressing CpG motifs (CpG-ODN) mimic the immunostimulatory activity of bacterial DNA and are commonly used to activate Toll Like Receptor 9 (TLR9) for therapeutic applications. CpG-ODN can counteract the activity of DNA-damaging chemotherapy and radiation therapy on immune cell and enhance immunity in preclinical mouse models. We hypothesized that these actions of CpG-ODN on immune cells are … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 27 Jul 2016 · 7,625 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleTelomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age PredictionNaglaa F. Alhusseini, Abdelmonem G. MadbolyThe fact that telomere shortening occurs with aging leads to the hypothesis that a correlation could be made between Telomere Length (TL) and suspect age. This study aimed to assess if TL can be used as an investigational tool to predict human age through developing a formula based on this correlation with accuracy suitable to be applied in forensic practice. … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 24 Mar 2016 · 8,004 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease VirusAsmarani Kusumawati, Tenri A. Wanahari, Issabellina D. Tampubolon, Basofi A. MappakayaJembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 26 Jun 2015 · 7,798 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleA NEW PCR-RFLP FOR SPECIES-SPECIFIC DIAGNOSIS OF SOUTH AMERICAN ANIMAL TRYPANOSOMIASISLiliana Torcoroma García, Yezid A. Ardila, Deilis Rincón et al.The diagnosis of animal trypanosomiasis in field samples is currently based on clinical suspicion and less frequently on traditional parasitological methods characterized by their very low sensitivity, especially in the sub-acute and chronic phase of the infection. Molecular tools such as Polymerase Chain Reaction have been shown to reach the highest sensitivity percentages, but without good resolution for all of … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 2 · 5 Jun 2014 · 7,182 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGENOTYPIC IDENTIFICATION OF CANDIDA SPP. ISOLATED FROM ONYCHOCANDIDIASIS PATIENTS BY PHENOTYPIC METHODS, PCR AND RAPD-PCRZaidan Khlaif Imran, H. Al-GhalibiCandidal Onychomycosis (COM) is a common nail disease which plays as sources pathogenic reservoir giving a rise to repeated candidiasis infections. This study aimed to evaluate PCR assays and phenotypic tests for identification of yeasts isolated from COM patients. The study included 100 clinically suspected patients of COM attending the main hospital and clinics in Al-Dewania province in the middle … …Current Research in Medicine, Vol. 5, Issue 1 · 31 Dec 2013 · 7,263 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRapid Real Time PCR Based Detection of Cell Count in Case of Urinary Tract InfectionPoulomi Nandy, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriMicrobial identification and antimicrobial susceptibility testing methods currently used in clinical microbiology laboratories require at least two to three days because they rely on the growth and isolation of micro-organisms. This long, but necessary, delay has enormous consequences on prophylactic usage of antimicrobial drugs. This study was an attempt to reduce this detection time span. Taq Man Real Time PCR … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 22 Jun 2013 · 8,214 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePrevalence Study of Coxiella burnetii in Aborted Ovine and Caprine Fetuses by Evaluation of Nested and Real-Time PCR AssaysFarhad Safarpoor DehkordiProblem statement: Q fever is a ubiquitous zoonosis caused by Coxiella burnetii, an obligate intracellular rickettsial organism that caused abortion and stillbirth in ruminants. Approach: The prevalence of Coxiella burnetii in Iran is essentially unknown. Its traditional diagnosis is based on culture, serology and conventional PCR. In this present study, for more sensitive and accurate detection and prevalence's determination of … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 4 · 13 Jan 2012 · 8,994 accessesView PDFCite