Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)
Lesley Maurice Bilung1, Son Radu2, Abdul Rani Bahaman2, Raha Abdul Rahim2, Suhaimi Napis2, Cheah Yoke Kqueen2, Chandrika Murugaiah2, Yousr Abdul Hadi2, Tunung Robin2 & Mitsuaki Nishibuchi3 Show affiliations
- University Tunku Abdul Rahman, Malaysia
- University Putra Malaysia, Malaysia
- Kyoto University, Malaysia
American Journal of Immunology, Volume 1, Issue 1, pp. 31–36 · doi.org/10.3844/ajisp.2005.31.36
Abstract
Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.
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Bilung, L. M., Radu, S., Bahaman, A. R., Rahim, R. A., Napis, S., Kqueen, C. Y., Murugaiah, C., Hadi, Y. A., Robin, T. & Nishibuchi, M. (2005). Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa). American Journal of Immunology, 1(1), 31–36. https://doi.org/10.3844/ajisp.2005.31.36
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