Sign inSubmit
Tips: “quotes” for a phrase · author:Lee · a DOI goes straight to its article

28 results for “Flow Cytometry”
Set a search alert
Research ArticleOpen accessIn the abstractTaxifolin Potently Diminishes Levels of Reactive Oxygen Species in Living Cells Possibly by Scavenging Peroxyl RadicalsSatoru Sakuma, Yui Kishiwaki, Mayu Matsumura et al.Taxifolin, a naturally occurring dihydroflavonol, has been reported to possess antioxidant activity, whereas there is little information regarding its antioxidant potency as a flavonoid. In the present study, the antioxidant efficacy of taxifolin in living cells was compared to that of catechin, a potent radical scavenger, by using flow cytometry with the redox-sensitive fluorescent dye, 2′,7′-dichlorodihydrofluorescein diacetate. Further, diminishing efficacy … …American Journal of Pharmacology and Toxicology, Vol. 13, Issue 1 · 18 Oct 2018 · 8,058 accessesView PDFCite
Research ArticleOpen accessIn the abstractChronic Mental Stress Induces Reversible Reduction of Natural Killer Cells and CD56dim SubpopulationManar M. IsmailGenerally, studying to be a health care provider is a stressful and demanding field and the students have to face many stressors that may affect their general health status including the immune system. This work aimed at studying the effect of prolonged naturalistic life-stress exposure on the percentage of peripheral blood T-lymphocytes and Natural Killer (NK) cells in female laboratory … …American Journal of Immunology, Vol. 13, Issue 3 · 30 Aug 2017 · 7,794 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine NeutrophilsSuphakit Srikok, Sukij Nambut, Kanruethai Wongsawan, Phongsakorn ChuammitriThis study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 2 Aug 2017 · 8,124 accessesView PDFCite
Research ArticleOpen accessIn the abstractHumoral and Cellular Effects of Stress-An Extensive Model SystemMazal Shimon-Hophy, Ramy R. AvtalionThe influence of stress on the immune system of the common carp (Cyprinus carpio) was studied by measuring leukocytes levels using flow cytometry and mRNA immune components by real time qPCR. Acute and chronic oxidative stresses were generated by different regimes of exposure of carp to environmental air. In acute stress, induced by single air exposure, the pro-inflammatory cytokines (IL1β, … …American Journal of Immunology, Vol. 13, Issue 2 · 18 Apr 2017 · 6,728 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmunologic Aspect of Mice (Mus musculus) Dental Caries Induced by Streptococcus mutansSri MurwaniDental caries (tooth decay), cavities or caries is a breakdown of teeth due to activities of bacteria acid producing in the presence of fermentable carbohydrates. The most responsible bacteria for dental cavities are Streptococcus mutans. Dental shape, saliva pH, normal flora, diets were suggested as predisposition factors for dental plaque and caries. The aim of this research was to study … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 1 · 28 Mar 2017 · 8,128 accessesView PDFCite
Research ArticleOpen accessIn the abstractPotential of HMEC-1 Line and HUVEC Primary Culture Cells to Study the Neonatal IgG Fc Receptor in vitroLuz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Felipe Vadillo-Ortega et al.The neonatal IgG Fc receptor (FcRn) plays an important role in IgG homeostasis and immunity passive transfer. Fine points regarding these mechanisms, however, are still emerging. In order to obtain information about these phenomena, it is essential to have in vitro models of endothelium that express this receptor. In this study we chose two widely used models of human endothelial … …American Journal of Immunology, Vol. 12, Issue 1 · 21 Mar 2016 · 9,455 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Short Term in vitro Stress Hormone Exposure on Regulatory T Cell Number and Function in AsthmaKristina E. Rehm, Gailen D. Marshall, Jr.Asthma is characterized by alterations in the immune system, including regulatory T cells (Treg). Further alterations in Treg numbers and/or function caused by stress hormones may be a contributing factor in asthma pathogenesis. We compared Treg populations and the effects of Dexamethasone (DEX, a laboratory analog of cortisol) on Treg cell number and function in patients with asthma and a … …American Journal of Immunology, Vol. 11, Issue 1 · 24 Apr 2015 · 6,610 accessesView PDFCite
Research ArticleOpen accessIn the abstractCYTOTOXICITY AND MODE OF CELL DEATH INDUCED BY TRIPHENYLTIN (IV) COMPOUNDS IN VITRONormah Awang, Zalila Abdul Aziz, Nurul Farahana Kamaludin, Kok Meng ChanA series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. … …OnLine Journal of Biological Sciences, Vol. 14, Issue 2 · 15 Mar 2014 · 8,750 accessesView PDFCite
Research ArticleOpen accessIn the abstractMelanoma-Associated Suppression of the Dendritic Cell Lines DC2.4 and JawsiiKristian Michael Hargadon, Yonathan Tarekegne Ararso, Osric Anthony Forrest, Chad Mason HarteDendritic cells function as potent regulators of both innate and adaptive immunity to tumors and the regulatory activities of these cells are tightly linked to their maturation and activation status. Despite the critical role played by dendritic cells in the induction of anti-tumor immune responses, the number of dendritic cells that can be isolated from experimental animals is limiting and … …American Journal of Immunology, Vol. 8, Issue 4 · 3 Nov 2012 · 9,567 accessesView PDFCite
Research ArticleOpen accessIn the abstractElevated Th17 and IL-23 in Hypertensive Patients with Acutly Increased Blood PressureJunxian Wang, Jianying Chen, Can Chen et al.Problem statement: Many severe cardiovascular complications such as stroke and heart attack can result from acutely increased blood pressure in patients with hypertension. The underlying mechanisms remain unclear despite of extensive study. To characterize the inflammatory mechanisms in hypertensive patients with acute blood pressure increase, we tested circulating Th17 levels, IL-23R expression and plasma levels of IL-17 and IL-23 in … …American Journal of Immunology, Vol. 8, Issue 2 · 21 May 2012 · 7,920 accessesView PDFCite