28 results for “Flow Cytometry”

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Research ArticleOpen accessPhrase in the titleDevelopment of an Immunomagnetic Separation Method for Viable Salmonella Typhimurium Detected by Flow CytometryShakil Ahmed, Horst-Günter Rubahn, Helmut ErdmannA very small number of bacterial pathogens may have fatal effects on food safety. In spite of having great advancements in bioanalytical methods, most of the accepted detection methods are still cultivation based and thus time consuming. This leads to an intense need for efficient and rapid methods for detection of food-related bacteria. In this study, a flow cytometry based … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 25 Nov 2016 · 7,578 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleFlow Cytometry Baseline on Peripheral Leukocyte Cell Profiles for Dairy Goat KidsDarcy M. Fletcher, Megan B. Vogt, Hannah E. Pirner et al.Small ruminants, like goats, would make excellent animal models for not only infectious diseases in large ruminants but also analogous diseases in humans, such as human tuberculosis, Crohn’s disease, melioidosis and brucellosis. The main disadvantage for the small ruminant model is the lack of sufficient baseline data on normal, healthy goat kids. Furthermore, most reagents (antibodies and the like) were … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 4 · 26 Sep 2013 · 7,576 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsBlood-Circulating Type 2 Follicular Helper T Cells in Pediatric Allergy PatientsMasaaki Hamada, Yoshihiko Sakurai, Tomohiro TakedaPediatric allergic diseases are primarily caused by an IgE-dependent immunological reaction. Despite studies reporting the involvement of T follicular Helper (TfH) cells, especially type 2 TfH cells, in class-switching to IgE production in B cells, TfH subset skewing in peripheral blood in pediatric allergy patients remains to be elucidated. This study aimed to investigate the possible involvement of type 2 … …American Journal of Immunology, Vol. 16, Issue 1 · 18 Jun 2020 · 7,506 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsFLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA BETWEEN OKRA LANDRACES (ABELMOSCHUS ESCULENTUS L.)Naser M. SalamehOkra Abelmoschus esculentus L. (Moench), is an economically important vegetable crop grown in tropical and sub-tropical as well as Mediterranean countries. The genome size of the Abelmoschus genus species is still rare. Ploidy determinations have traditionally been done by counting chromosomes of stained root tips, but this method is laborious and often difficult with species which have small chromosomes and … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 2 · 13 Feb 2014 · 8,090 accessesView PDF Cite

Short CommunicationsOpen accessIn the keywordsCytotoxicity and Apoptosis-Induction Potential of Fraction F3 from Fruits of Bersama engleriana (Melanthaceae) against Human Cervical Cancer CellsMichèle Stella Majoumouo et al.Cervical cancer is one of the most life-threatening malignancies worldwide, despite intensive research for more efficacious chemotherapeutic agents. Recent efforts geared towards eradicating the scourge of cancer have embodied a major surge of interest in natural products as promising lead molecules in pharmaceutical development and therapeutic translation. Consequently, this study aimed to assess the cytotoxicity of a previously identified plant … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 5 Dec 2022 · 5,294 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEvaluation of Anti-Inflammatory Potential of Turmimax® an in vivo and in vitro StudyFiroz Hussain H M., Nanjundaiah S., Sadashiva Channangihalli Thimmegowda et al.The main objective of this study was to investigate the in vivo and in vitro anti-inflammatory effects of the Turmimax® bio-enhanced turmeric formulation. By comparing the relative fluorescence intensities of the interleukins IL-8 (Interleukin-8) and IL-12 (Interleukin-12) cytokines in stimulated murine Raw 264.7 macrophages, Turmimax® was evaluated for its anti-inflammatory in vitro efficacy. Turmimax® was administered orally in two doses, … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 24 Nov 2022 · 6,587 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsCo-expression of Apoptosis-Related Molecules on Activated CD8+ CD38+ T-cells is Associated with HIV-1 Disease ProgressionJosé W. Rodríguez, Luis A. Rodríguez, Griselle Font et al.CD8+ T cells play a major role in controlling HIV-1 infection through the release of soluble lytic and non-lytic antiviral factors. Their decrease or defective function contributes to the HIV-1 disease progression. HIV-1 disease progression has been associated with a remarkable increase of CD38 expression on CD8+ T-cells. It has been also documented that a significant distribution of HIV-specific CD8+T-cells … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 7,016 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEffects of Atorvastatin on Oxidized Low-Density Lipoprotein-induced Proliferation, Apoptosis, and Matrix Metalloproteinase-9 Expression in Human MacrophagesBensheng Liu, Huifan Li, Yan Wang, Li ZhangAtorvastatin (ATV), an HMG-CoA reductase inhibitor, is utilized in the treatment of atherosclerotic diseases. Mononuclear Macrophages (MMs) are critical immune cells involved in atherosclerosis progression, participating in processes such as foam cell formation, cytokine secretion, and the development of fibrous cap and lipid core. To further elucidate the mechanism by which ATV exerts its therapeutic effects on atherosclerosis through the … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 3 · 18 Nov 2025 · 2,136 accessesView PDF Cite

Research ArticleOpen accessIn the abstractRadiation Triggered Phenotypic Changes of Regulatory T CellsLantao Liu, Yan Peng, Deqin Zhang, Dongsheng NiuIonizing radiation disrupts the immune balance easily. Regulatory T (Treg) cells directly affect the immune balance after radiation exposure. Meanwhile, the present study is to investigate the survival ability and phenotypic changes of Treg cells induced by 60Co γ-rays in order to expand the understanding on how Treg cells modulated by radiation. Mice lymphocytes were isolated from thymus and spleen … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 20 Mar 2022 · 5,830 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInfluence of Different Freezing Curves on the Acrosome Integrity of Male Goat Sperm CellsSofia Alexandra Fagundes Lopes, Henrique José Duarte Rosa, António Chaveiro, Fernando Moreira da SilvaThe aim of the present study was to evaluate the effect of three different freezing curves on male goat’s sperm viability and acrosome integrity. Semen was collected on three adult male goats in February (Azores, Portugal, latitude 38°39′N) using an artificial vagina and a female with induced heat. Three collection sessions were spaced of approximately 5 days. The study followed … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 19 Feb 2021 · 6,618 accessesView PDF Cite