14 articles for “DNA extraction”

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Research ArticleOpen accessIn the abstractPolymorphism of Insulin-Like Growth Factor 1 Gene in Najdi Cattle PopulationsAhmad Yazdanpanah, Khalil Mirzadeh, Morteza Mamouei et al.The Insulin-like Growth Factor 1 (IGF-1) system plays a critical endocrine role controlling nutrient metabolism in dairy cattle and it is a group of structurally-related polypeptides that regulate the growth of many types of mammalian cells. In liver, IGF-1 is dynamically regulated by lactation and energy balance. Less is known about the regulation of IGF-1 and IGF-binding protein mRNA in … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 7,154 accessesView PDF Cite

Research ArticleOpen accessIn the abstractISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTSGuetarni Hassina, Bensoltane AhmedThe objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the … …OnLine Journal of Biological Sciences, Vol. 13, Issue 2 · 19 Apr 2013 · 7,264 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetection of Listeria monocytogenes in Ready-to-Eat Meat ProductsAsma M.M.A. Abdelgadir, Kunwar K. Srivastava, P. Gopal ReddyProblem statement: The objective of this study was to develop a rapid and sensitive method for the detection of Listeria monocytogenes and to screen large number of ready-to-eat (RTE) meat samples. Approach: A total of 250 fresh RTE meat samples of different varieties (chicken, turkey, beef, pork and cold cuts) were purchased from local grocery stores. Results: From the 250 … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 30 Jan 2012 · 7,421 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGenetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native FowlsHossein Ali Abbasi, Shahabodin Gharavysi, Rohullah AbdullahpourProblem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 17 Sep 2011 · 7,700 accessesView PDF Cite