ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS
Guetarni Hassina1 & Bensoltane Ahmed2 Show affiliations
- University of Khemis Miliana, Algeria
- University of Oran Es-Senia, Algeria
OnLine Journal of Biological Sciences, Volume 13, Issue 2, pp. 41–49 · doi.org/10.3844/ojbsci.2013.41.49
Abstract
The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.
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Hassina, G. & Ahmed, B. (2013). ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS. OnLine Journal of Biological Sciences, 13(2), 41–49. https://doi.org/10.3844/ojbsci.2013.41.49
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