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14 results for “DNA extraction”
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Research ArticleOpen accessPhrase in the titleAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExtraction of Genomic DNA Using Magnetic Nanoparticles (Fe3O4) as a Solid-Phase SupportZ. M. Saiyed, C. N. RamchandMagnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 9,826 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGeneration of Five New Musa Hybrids With Resistance To Black Sigatoka and High YieldRicardo Hernández, Teresa Ramírez, Juan Carlos Noa-Carrazana et al.The ability to identify genetic variation is indispensable to effective management and use of genetic resources. This work is the first approach concerning to generation and genetic differentiation of new Musa hybrid lines obtained in INIVIT (Cuba), crossing Musa acuminata male diploid (AA) x female triploid (ABB), genotypes selected by their resistant character to pests and diseases. From the obtained … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 8,811 accessesView PDFCite
Research ArticleOpen accessIn the abstractHuman Papillomavirus Genotypes in the Korean Older Adult Population Running Head: HPV Genotypes in Korean Older AdultsJieun Hwang, Hee Seung Song et al.In older populations, Human Papillomavirus (HPV) infections are likely to reactivate owing to immunocompromised, thereby increasing the risk of cervical and other cancers. However, research on HPV infection in older adults is limited and data on the sex- and age-specific distribution of high-risk HPV types are lacking. Thus, to increase the level of evidence on the extension of cervical cancer … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 6 Dec 2024 · 5,007 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnthelmintic Treatment in Growing Cattle: A Preliminary Study on the Effect of Ricobendazole on the Fecal MicrobiomePablo Rovira, Pablo LorenzoThere is limited information on the impact that anthelmintic treatments exert on the fecal microbiome of growing cattle. This study characterizes the composition and metabolic function of the cattle gut microbiome associated with different levels of parasite Fecal Egg Counts (FEC) and characterizes the fecal microbiome before and after ricobendazole-treatment. The hypothesis was that FEC levels and ricobendazole treatment (8 … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 1 · 31 Mar 2022 · 6,340 accessesView PDFCite
Research ArticleOpen accessIn the abstractDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Characterization of a Bovine Breed (Tabapua) Under Artificial Selection Located in the Amazon RegionJose Elivalto Guimaraes Campelo, Marcone Helmer da Silva, Ednaldo da Silva Filho et al.Brazil currently has the biggest commercial bovine cattle population in the world. Several different breeds that come from Europe and India compose it. They are primarily used for dairy and meat production. In Brazil, several high quality genetic background cattle are kept in a condition known as pure breed, meaning no other animal of different genetic background is interbreeding with … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 30 Jan 2013 · 7,552 accessesView PDFCite
Research ArticleOpen accessIn the abstractHepatitis B Virus DNA Can be Amplified Directly From Dried Blood Spot on Filter PaperNaglaa Fathy Alhusseini, Mamdouh Z. Abadeer, Sheref M. El-TaherHepatitis B Virus (HBV) infections represent a major public health problem because of the ability of HBV to cause a chronic carrier state. Even though chronic carriers remain largely asymptomatic, a large number of these individuals subsequently develop cirrhosis and primary hepatocellular carcinomas. Dried Blood Spot (DBS) samples are a simple and inexpensive sampling method, especially useful for blood collection … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 8,454 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Feasibility Study of Challenges and Opportunities in Computational Biology: A Malaysian PerspectiveR. J.A. Richard, N. SriraamThe term computational biology refers to the knowledge derived from a computer analysis of biological data that includes identification of genes in DNA sequence of different organisms, prediction of structural and functional mechanism of proteins, feature extraction and classification of genomics and proteomics. Computational biology is a rapidly developing branch of science and is highly interdisciplinary, using techniques and Concepts … …American Journal of Applied Sciences, Vol. 2, Issue 9 · 30 Sep 2005 · 6,924 accessesView PDFCite
Research ArticleOpen accessIn the abstractPolymorphisms in the 5’ Flanking Region and First Exon of Insulin-Like Growth Factor 1 Gene in Southern Populations of BuffaloSaber Khederzadeh, Ahmad YazdanpanahThe Insulin-like Growth Factor 1 (IGF-1) system plays a critical endocrine role controlling nutrient metabolism in animals and it is a group of structurally-related polypeptides that regulate the growth of many types of mammalian cells. In liver, IGF-1 is dynamically regulated by lactation and energy balance. Less is known about the regulation of IGF-1 and IGF-binding protein mRNA in reproductive … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 6,846 accessesView PDFCite