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167 results for “ELISA”
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Research ArticleOpen accessIn the abstractReactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivoAurora Martinez-Romero, Jose Luis Ortega-Sanchez, Jose Ramon Hernandez-Salgado et al.Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 31 Mar 2009 · 7,617 accessesView PDFCite
Research ArticleOpen accessIn the abstractSerum Levels of Insulin-Like Growth Factor-I, Thyroid Hormones and Skeletal Muscle Fiber Size in Castrated Lambs with and Without Androgen TreatmentBani Z. Ismail, O. M. AL-Ababneh, M. B. AL-ZghoulTwelve, intact male lambs, 1-2- weeks old, were divided into 3 groups, Group W (4 lambs) were castrated at age less than 1 month, group WT (4 lambs) were castrated at the same age and treated with testosterone and group R (4 lambs) were left intact and served as control. Testosterone Propionate replacement was administered intramuscularly at a dose of … …American Journal of Applied Sciences, Vol. 6, Issue 3 · 31 Mar 2009 · 7,129 accessesView PDFCite
Research ArticleOpen accessIn the abstractEstimating HIV-1 Incidence Using the Serologic Testing Algorithm for Recent HIV Seroconversion at HIV Counseling and Testing Centers in the City of São Paulo, BrazilKatia Cristina Bassichetto, Denise Pimentel Bergamaschi, Maria Amelia Veras et al.The network of HIV counseling and testing centers in São Paulo, Brazil is a major source of data used to build epidemiological profiles of the client population. In this study, HIV-1 incidence from November of 2000 to April of 2001 was estimated, comparing recently infected individuals to those with long-standing infection in terms of epidemiological and socio-behavioral aspects. To identify … …American Journal of Infectious Diseases, Vol. 4, Issue 4 · 31 Dec 2008 · 6,463 accessesView PDFCite
Research ArticleOpen accessIn the abstractOxidative Stress in Autism: Elevated Cerebellar 3-nitrotyrosine LevelsElizabeth M. Sajdel-Sulkowska, Boguslaw Lipinski, Herb Windom et al.It has been suggested that oxidative stress and/or mercury compounds play an important role in the pathophysiology of autism. This study compared for the first time the cerebellar levels of the oxidative stress marker 3-nitrotyrosine (3-NT), mercury (Hg) and the antioxidant selenium (Se) levels between control and autistic subjects. Tissue homogenates were prepared in the presence of protease inhibitors from … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 2 · 30 Jun 2008 · 17,134 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Autistic Phenotype Exhibits a Remarkably Localized Modification of Brain Protein by Products of Free Radical-Induced Lipid OxidationTeresa A. Evans, Sandra L. Siedlak, Liang Lu et al.Oxidative damage has been documented in the peripheral tissues of autism patients. In this study, we sought evidence of oxidative injury in autistic brain. Carboxyethyl pyrrole (CEP) and iso[4]levuglandin (iso[4]LG)E2-protein adducts, that are uniquely generated through peroxidation of docosahexaenoate and arachidonate-containing lipids respectively, and heme oxygenase-1 were detected immunocytochemically in cortical brain tissues and by ELISA in blood plasma. Significant … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 2 · 30 Jun 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractEpitope Mapping of Dengue-Virus-Enhancing Monoclonal-Antibody Using Phage Display Peptide LibraryChung-I Rai, Huan-Yao Lei, Yee-Shin Lin et al.The Antibody-Dependent Enhancement (ADE) hypothesis has been proposed to explain why more severe manifestations of Dengue Hemorrhagic Fever and Dengue Shock Syndrome (DHF/DSS) occur predominantly during secondary infections of Dengue Virus (DV) with different serotypes. However, the epitopes recognized by these enhancing antibodies are unclear. Recently, antipre-M monoclonal antibody (mAb 70-21), which recognized all DV serotypes without neutralizing activity, were … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 8,455 accessesView PDFCite
Research ArticleOpen accessIn the abstractT-2 Toxin Regulated Ganoderma lucidum Induced Cytokine ReleaseKazem Ahmadi, Majid RiazipourThe water-soluble extract of Ganoderma lucidum (Reishi) has been used as immunomodulator to stimulate spleen cells proliferation and cytokine expression. It has also been shown that at some level of exposure, T-2 toxin typically act as immunosuppressive agent and can increase disease susceptibility. The aim of this study was to investigate the effect of T-2 toxin on cytokine production by … …American Journal of Immunology, Vol. 4, Issue 1 · 31 Mar 2008 · 7,541 accessesView PDFCite
Research ArticleOpen accessIn the abstractRelation between Autoimmunity Chronic Urticaria and the Levels of Plasma Prothrombin F1+2Liu Xiaoming, Sheng Wanxiang, Ahmad T. Khalaf et al.A number of recent epidemiologic and experimental studies have enhanced our knowledge of the etiology and pathogenesis of chronic urticaria (CU), a disease that imposes profound impairment on patient's quality of life. Our study was performed to explore the relation between autoimmunity (CU) and the levels of plasma prothrombin F1+2. Thirty four (CU) patients with positive autologous serum skin test … …American Journal of Applied Sciences, Vol. 4, Issue 12 · 31 Dec 2007 · 6,900 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Value of Serologic Tests for Diagnosis and Follow up of Patients having BrucellosisNidia E. Lucero, Sandra M. Ayala, Gabriela I. Escobar, N R. JacobThough diagnosis of human brucellosis is accurate when the causal agent is isolated, this procedure is not always successful and the most of patients are diagnosed on the basis of rising titres of antibodies in serum. The classical tests used for detection of antibodies to S-Brucella sp., include Rose Bengal (RBT), buffered plate antigen (BPAT), serum agglutination (SAT), 2-mercapto-ethanol (2MET) … …American Journal of Infectious Diseases, Vol. 3, Issue 1 · 31 Mar 2007 · 7,447 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of a DNA Vaccine for Immunocontraceptive Potential Against Zona Pellucida Glycoproteins in CattleC.A. Foley, S.M. Boyle, R.E. Pearson, F.C. GwazdauskasHolstein cows were administered zona pellucida (ZP) DNA vaccine and used to determine the potential of recombinant rabbit ZP glycoproteins (rZP) as immunocontraceptive antigens. Zona pellucida proteins were purified and quantified. Cows were assigned to one of four treatment groups in which plasmids encoding rabbit ZP proteins were administered, i.d., using a gene gun (ZP55, n=2; ZP75, n=2; Hep55, n=2; … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,890 accessesView PDFCite