Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo

Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO_2^- and NO_3^-) were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). Results: The treatment with the 1, 25(OH)₂D3 stimulated the NO₃^- synthesis indicating, that by itself it is a good modulador of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)₂D3. The exhibition to M. bovis-BCG vaccine with the treatment with 1, 25(OH)₂D3 was able to increase answer NO₃^- in exposed animals. Conclusion: The 1, 25(OH)₂D3 stimulated in vivo the production of RNI in goats exposed to M. bovis BCG vaccine.