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147 results for “Chromatography”
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Research ArticleOpen accessIn the abstractThe Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma, Cheng Hock ChuahThis study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using … …American Journal of Applied Sciences, Vol. 4, Issue 6 · 30 Jun 2007 · 8,362 accessesView PDFCite
Research ArticleOpen accessIn the abstractPreparation and Properties of Copolyanhydrides Based on 2-Hexadecylsuccinic Acid and Sebacic AcidYousef Hamdan, Xiangmei Jiang, Kaixun Huang, Kaichao YuPoly(2-hexadecylsuccinic acid-sebacic acid) (P(HSA-SA)) copolymers have been prepared by melt polycondensation. The copolymers were characterized by FT-IR, 1HNMR, gel permeation chromatography (GPC), differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). In vitro studies showed that all our prepared copolymers are degradable in phosphate buffer at 37°C. The release profiles of hydrophilic model drug, ciprofloxcin hydrochloride, from the copolymers, follow … …American Journal of Applied Sciences, Vol. 4, Issue 3 · 31 Mar 2007 · 7,427 accessesView PDFCite
Research ArticleOpen accessIn the abstractSeparation of Guanine and Hypoxanthine with Some Ionic Liquids in RP-HPLCJ. Zheng, K. H. RowIn this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RP-HPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-octyl-3-methylimidazolium methylsulfate ([OMIm][MS]) were used. Guanine and hypoxanthine couldn’t be separated with many different kinds of unadjusted mobile phase, such … …American Journal of Applied Sciences, Vol. 3, Issue 12 · 31 Dec 2006 · 6,892 accessesView PDFCite
Research ArticleOpen accessIn the abstractLarge Scale Production and Characterization of Anti-Human IgG Monoclonal Antibody in Peritoneum of Balb/c MICEB. Baradaran, J. Majidi, Z. M. Hassan, J. AbdolalizadehMonoclonal antibodies are key reagents that are used in biomedical researches, diagnosis of immunodeficiency diseases such as IgG subclasses deficiency and treatment of diseases like infections and cancers. For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human IgG were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 4 · 31 Dec 2005 · 7,089 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,913 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effects of Chinese Traditional Processing Method on Components in Semen Sinapis AlbaeLifang Liu, Hui Zhou, Suqin Sun et al.Techniques of infrared (IR) spectrophotometry, High Performance Liquid Chromatography (HPLC) and HPLC-mass spectrometry are employed in this study to reveal the changes of the components in Semen sinapis Albae during the Chinese traditional processing. While the degradation of proteins and polysaccarides can be observed, sinapine in this drug can be known to decompose immediately after 15 mins during processing. A … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,810 accessesView PDFCite
Research ArticleOpen accessIn the abstractMethyltert-butyl Ether (MTBE) Degradation by a Microbial ConsortiumS. B. Mortazavi, A. Nikpey, A. Rezaee et al.Methyl tert-butyl ether (MTBE) is added to reformulated gasoline to meet the 1990 Clean Air Act directives. Widespread use of MTBE in gasoline has resulted in groundwater contamination. Because of its undesirable effects on drinking water and ecologically harmful effects, MTBE removal has become a public health and environmental concern. In this study, we have isolated a mixed bacterial culture … …American Journal of Environmental Sciences, Vol. 1, Issue 1 · 31 Mar 2005 · 6,994 accessesView PDFCite