Research ArticleOpen accessIn the keywordsProteomics Approach for Investigating the Disease Resistance Using Cucumber as Model PlantSaligrama A. Deepak, Junko Shibato, Hideo Ishii et al.The profile of proteins induced in cucumber leaves pretreated with Acibenzolar-S-Methyl (ASM) and challenge inoculated with Colletotrichum orbiculare are presented. ASM was applied to the first leaf and the third leaf collected at different time intervals of 0-48 h and the total protein extracted using trichloroacetic acid acetone was analyzed by two-dimensional gel electrophoresis. Increased expressions of pyridoxine biosynthesis protein, … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 7,240 accessesView PDFCite
Research ArticleOpen accessIn the abstractA common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case StudyJyoti Bhardwaj, Sudesh Kumar YadavThree protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 28 Oct 2013 · 7,925 accessesView PDFCite