3 results for “serum-free medium”

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Research ArticleOpen accessPhrase in the titleBio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free MediumTereza C. Cardoso, Heitor F. Ferrari, Maria Cecília R. Luvizotto, Clarice W. ArnsThe detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 6,991 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleApplication of a Serum/Protein-Free Medium for the Growth of Mammalian Cell Lines and High Level Production of Classical, Variant and Very Virulent Strain of Infectious Bursal Disease Virus (IBDV)Ane Franciele Tapparo, Paloma Oliveira Tonietti, Maria CecÍlia R. Luvizotto, Tereza C. CardosoThe aim of this study was to verify the capacity of IBDV strains, Lukert, F52/70 and very virulent Brazilian genotype (G11) to infect and replicate on cell culture maintained without serum or protein supplementation. BHK21 and CER cells were directed adapted to growth under absence of protein and/or fetal bovine serum with addition of 5 µg mL-1 of insulin-like growth … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 7,656 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDevelopment of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free ConditionsFrancesca Abramo, Andrea Pirone, Carla Lenzi et al.Full-thickness canine skin organ culture models could provide an entirely new opportunity for studying wound healing, keratinization disorders and allergic skin diseases, all of which have high prevalence and severe impact on canine quality of life. Here we present a canine organ culture method for the short-term maintenance of full-thickness, adult, canine skin in serum-free medium and investigate the possibility … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 2 · 13 Jun 2016 · 7,380 accessesView PDF Cite