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17 articles for “recombinant enzyme”
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Research ArticleOpen accessEvery word in the titlePurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Cyanobacterium Synechocystis sp. PCC 6803 Glutaredoxin Gene (slr1562) Protects Escherichia coli against Abiotic StressesAhmed Gaber, Salah El-Din El-AssalProblem statement: Glutaredoxins (GRXs) are ubiquitous small heat stable glutathione-dependent oxidoreductase enzymes that play a crucial role in plant development and response to oxidative stress. Approach: Cyanobacterium Synechocystis strain PCC 6803 contains two genes (slr1562 and ssr2061) encoding glutaredoxins. In the present investigation the slr1562 gene (grxC) was isolated and characterized. Results: The results revealed that the amino acid sequence … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 1 · 5 Mar 2012 · 7,582 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning and Expression of SCAMP3 in Transgenic Silkworm (Bombyx mori)Selly S. Rajagukguk, Sabar Pambudi, Astari Dwiranti et al.SCAMP3 (Secretory Carrier Membrane Protein 3) is a crucial component of vesicular trafficking pathways and plays important roles in endocytosis and protein recycling. Previous studies demonstrate SCAMP3 dysregulation in various cancers, suggesting potential applications as a biomarker and therapeutic target. Understanding SCAMP3 expression and post-translational processing provides insights into its biological functions and potential applications in cancer diagnostics and therapy. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 16 Feb 2026 · 1,521 accessesView PDFCite
EditorialOpen accessIn the abstractCommentary-How do we Improve Training for Receptor Research?Marilee Ann BenoreThis issue of the American Journal of Biochemistry and Biotechnology features articles about receptor-ligand interactions that are critical to the wellbeing of all living organisms. The importance of the field of receptor research is supported by the large number of articles published in journals, the numerous specialty journals, the international conferences and the percentage of legal drugs on the market … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 4 · 8 Dec 2015 · 8,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03Viniti Vaidya, Gajjeraman Prabu, D. Theertha PrasadThermostable levansucrase encoding gene (sacB) with its promoter was isolated from Bacillus subtilis BB03. BsSacB is composed of 1419 bp, encoding 473 amino acid residues and with promoter regions TTGCAA (-35) and TAGAAT (-10). The amino acid sequence analysis of BsSacB showed conserved motifs of microbial fructosyltransferase belonging to Glycoside Hydrolase family 68 (GH68). Comparative analysis of the protein structure … …OnLine Journal of Biological Sciences, Vol. 15, Issue 1 · 25 Mar 2015 · 7,371 accessesView PDFCite
Research ArticleOpen accessIn the abstractProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,160 accessesView PDFCite
Research ArticleOpen accessIn the abstractInduced Production of α-Tocotrienol by Co-Suppression of Tocopherol Cyclase Gene in Vegetable CropsUmaiyal Munusamy, Siti Nor Akmar Abdullah, Maheran Abdul Aziz et al.This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/Tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,706 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Seroepidemiological Survey of Hantavirus in Ilheus CountyMarcos Lazaro Moreli, Vivaldo Gomes da Costa, Flavia Rubia ParizHantaviral diseases are infections caused by a group of viruses that belong to the genus hantavirus and the family Bunyaviridae. In Brazil, several cases of hantavirus have been detected with the use of serological methods including immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA). To date, 1.573 cases have been reported in several states across the country with a fatality rate of … …Current Research in Virology, Vol. 1, Issue 1 · 30 Aug 2012 · 13,382 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Biodiesel by Enzymatic Transesterification: ReviewA.E. Ghaly, D. Dave, M.S. Brooks, S. BudgeProblem Statement: The research on the production of biodiesel has increased significantly in recent years because of the need for an alternative fuel which endows with biodegradability, low toxicity and renewability. Plant oils, animal fats, microalgal oils and waste products such as animal rendering, fish processing waste and cooking oils have been employed as feedstocks for biodiesel production. In order … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 21,467 accessesView PDFCite