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16 results for “real-time polymerase chain reaction”
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Research ArticleOpen accessPhrase in the titleDNA Code Word Design for DNA Computing with Real-Time Polymerase Chain ReactionMuhammad Faiz Mohamed Saaid, Zuwairie Ibrahim, Mohamad Shukri Zainal Abidin et al.Problem statement: A number of DNA computing models to solve mathematical graph problem such as the Hamiltonian Path Problem (HPP), Traveling Salesman Problem (TSP), and the Shortest Path Problem (SPP), have been proposed and demonstrated. Normally, the DNA sequences used for the computation should be critically designed in order to reduce error that could occur during a computation. We have … …Journal of Computer Science, Vol. 5, Issue 1 · 31 Jan 2009 · 7,506 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleApplications of Real-time Reverse Transcription Polymerase Chain Reaction in Clinical Virology Laboratories for the Diagnosis of Human DiseasesR. Manojkumar, V. MrudulaEarly diagnosis that prevents further expansion of the causative agent and thereby aids control and eradication of infectious agents is critical for countries trying to obtain a particular disease free status. In these diagnostics speed is paramount, including the assay’s applicability, sensitivity, specificity, cost effectiveness and patentability. Real-time Reverse Transcription PCR (rRT-PCR) has revolutionized the field of molecular biology and … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 6,557 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Underestimated Prevalence of Ureaplasma Infections: A Comprehensive Epidemiological Study in South Korea (2018-2020)Jae Eun Choi, Bo Kyeung Jung, Eun Ju Oh, Jae Kyung KimUreaplasma infections are frequently underestimated due to potential inaccuracies in data collection from symptomatic patients. This study aimed to accurately assess the prevalence of Ureaplasma Urealyticum (UU) and Ureaplasma Parvum (UP) infections among outpatients aged 0-70 years in South Korea between 2018 and 2020. A total of 59,381 clinical samples, including swabs and urine specimens, were collected and analyzed using … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 25 Mar 2024 · 6,137 accessesView PDFCite
Research ArticleOpen accessIn the abstractTrends in the Prevalence of Clostridioides difficile Using Real Time PCR in South Korea (2020-2024)Yonghee Lee, Yoo Na Chung, Jae Kyung KimClostridioides difficile is a major cause of infection in healthcare settings, and frequent antibiotic use exacerbates its occurrence. C. difficile infection (CDI) is a major public-health challenge owing to its complications and high mortality rate. We aimed to analyze current trends in C. difficile prevalence. This study was a retrospective, single center analysis of 7,371 samples from April 2020 to … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 3 Oct 2025 · 2,363 accessesView PDFCite
Research ArticleOpen accessIn the abstractHuman Papillomavirus Genotypes in the Korean Older Adult Population Running Head: HPV Genotypes in Korean Older AdultsJieun Hwang, Hee Seung Song et al.In older populations, Human Papillomavirus (HPV) infections are likely to reactivate owing to immunocompromised, thereby increasing the risk of cervical and other cancers. However, research on HPV infection in older adults is limited and data on the sex- and age-specific distribution of high-risk HPV types are lacking. Thus, to increase the level of evidence on the extension of cervical cancer … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 6 Dec 2024 · 5,019 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of the Utility of MPT64, Drug Susceptibility Testing and Tuberculosis/Nontuberculous Mycobacteria Reverse Transcription Polymerase Chain Reaction for Tuberculosis Diagnosis and Efficiency Compared with Traditional CultureHo Keun Choi, Ga Yeon Kim, Tae Soung Kim, Jae Kyung KimDefining the molecular mechanisms underlying the dormant state of Mycobacterium tuberculosis (MTB) is essential for developing more effective treatments for tuberculosis (TB) infection. We investigated the positivity and resistance rates of TB tests on 6,174 samples referred to a single hospital. Of these, 342 cases (5.5%) were confirmed as positive by acid-fast bacilli staining, 243 cases (71.1%) were detected as … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 5 Dec 2024 · 4,211 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Diarrhea-Causing Pathogens in South Korea Analyzed Using Molecular Diagnostic MethodsYonghee Lee, Jae Kyung KimGastrointestinal inflammation remains a prevalent global health concern. Prompt and accurate diagnosis of the condition is required to mitigate health risks in immunocompromised individuals and alleviate the economic burden linked to treatment and pathogen isolation. Challenges posed by traditional culture tests, such as long detection time and inability to isolate low-abundance organisms, can be overcome using molecular diagnostic methods, which … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 25 Jul 2024 · 5,012 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Plasma Amyloid-Beta and Apolipoprotein E Genotypes as Markers of Alzheimer’s Disease in Healthy Korean AdultsEun Ju Oh, Jung Jae Lee, Bo Kyeung Jung, Jae Kyung KimThe presence of the ApoE4 allele interferes with human Amyloid-Beta (Aβ) clearance and reduces the neuronal damage response, which, in turn, is associated with increased Alzheimer’s Disease (AD) risk. Identifying individuals with the highest genetic risk may aid clinical AD diagnosis and prognosis. However, to the best of our knowledge, no previous study has performed blood-based analysis of ApoE polymorphisms … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 26 Mar 2024 · 4,495 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssociation Between Cancer Screening Results Using Single Nucleotide Polymorphism and Cancer Incidence Rate in Korean WomenKyung Bae Lee, Jae Kyung KimIn this study, we investigated the usefulness of Single Nucleotide Polymorphism (SNP) testing in assessing cancer susceptibility in women by evaluating correlations between SNP testing results and cancer incidence rates. SNP testing was conducted on blood samples of women who underwent health examinations commissioned by medical institutions in the Republic of Korea in 2017 using real-time polymerase chain reaction with … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 25 Sep 2023 · 4,958 accessesView PDFCite
Research ArticleOpen accessIn the abstractHibiscus Flower Extract and Niacinamide Show Enhanced Whitening Effects in Mouse Melanoma Cells (B16F10)Jae Eun Choi, Tae Su Jang, Jung Min Park et al.Melanoma, a type of cancer that most often occurs on skin exposed to UV rays, is becoming increasingly common. Recent research has focused actively on using natural products. The goal of this study was to compare the melanin-inhibitory effects of Hibiscus (Hibiscus sabdariffa L.) flower Extract (HE) and Niacinamide (NA), a functional ingredient with whitening effects, on melanoma cells. The … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 9 Feb 2023 · 7,232 accessesView PDFCite