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35 results for “plasmid”
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Research ArticleOpen accessEvery word in the titleProfiling and Curing from Shigella Spp Isolated from Plasmid Diarrheal PatientsMunim R. Ali, Raad N. Hasan, Ali I. Mohammed, Ahmaed A. AbbasProblem statement: Shigellosis is a common infectious disease especially in underdeveloped countries. The bacteria are primarily transmitted through the faecal-oral route. The inflammatory process of acute Shigella infection affects the colon and is characterized clinically by fever, cramping abdominal pain with frequent loose stools that might contain mucus, pus and blood. Approach: This study determined the susceptibility of Shigella isolates … …Current Research in Microbiology, Vol. 1, Issue 1 · 30 Jun 2010 · 8,429 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDegradative Plasmid and Heavy Metal Resistance Plasmid Naturally Coexist in Phenol and Cyanide Assimilating BacteriaBahig El. Deeb, Abdullah D. AltalhiProblem statement: Heavy metals are known to be powerful inhibitors of xenobiotics biodegradation activities. Alleviation the inhibitory effect of these metals on the phenol biodegradation activities in presence of heavy metals resistant plasmid was investigated. Approach: Combination of genetic systems of degradation of xenobiotic compound and heavy metal resistance was one of the approaches to the creation of polyfunctional strains … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 11,984 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePlasmid Mediated Tolerance and Removal of Heavy Metals by Enterobacter spBahig El-DeebProblem statement: The role of plasmid in the heavy metal resistance and accumulation by endophytic bacteria was investigated. Approach: The experimental results showed that high level plasmid mediated Cd2+ and Zn2+ resistance in this strain is due to decreased Cd2+ and/or Zn2+ uptake/accumulation by resistance strain. Results: Based on the fact that subsequent plasmid curing experiments demonstrated that the ability … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 1 · 31 Mar 2009 · 8,001 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRole of Plasmid in Production of Acetobacter Xylinum BiofilmsAbbas Rezaee, Sanaz Solimani, Mehdi ForozandemogadamAcetobacter xylinum has the ability to produce cellulotic biofilms. Bacterial cellulose is expected to be used in many industrial or biomedical materials for its unique characteristics. A. xylinum contains a complex system of plasmid DNA molecules. A 44 kilobases (kb) plasmid was isolated in wild type of A. xylinum. To improve the cellulose producing ability of A. xylinum, role of … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 7,498 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA GenesTouati Abdelaziz, Mairi AssiaThe aim of this study is to make comparison of IncL/M groups on basis of two genes candidates’ repA and excA genes. The sequences of 27 plasmids were compared using the neighbor-joining method. This method was used to construct a phylogenetic tree for the nucleotide sequences of two genes (repA and excA), using the program MEGA X software. The evolutionary … …Current Research in Bioinformatics, Vol. 8, Issue 1 · 27 Feb 2020 · 7,088 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePlasmid-mediated Detoxification of Mycotoxin Zearalenone in Pseudomonas Sp. ZEA-1Abdullah D. AltalhiThe Pseudomonas sp. Strain ZEA-1 was isolated from rhizosphere of corn plant by an enrichment technique showed capability of utilizing zearalenone as the sole source of carbon. The bacterium rapidly utilized zearalenone beyond 200 µg/ml and showed prolific growth in a minimal medium containing 100 µg/ml zearalenone source. The course of ZEA degradation as well as the formation of its … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,854 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUltrastructural and Immunological Characterization of Hepatitis C Core Protein-DNA Plasmid ComplexesNelson Acosta-Rivero, Yaraima Aguilera, Viviana Falcon et al.Recently, it has been shown that a truncated HCV core (HCcAg) variant, covering the first 120 aa (HCcAg.120), interacts with plasmid DNA vaccine (pIDKE2), encoding the HCV structural proteins (HCcAg, E1 and E2). In the present work, HCcAg.120-pIDKE2 complexes, forming heterogeneous packaged structures, were visualized using a negative stain/rotary shadow technique. Interestingly, 72 hours after intramuscular injection of HCcAg.120-pIDKE2 complexes … …American Journal of Immunology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,743 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInteraction of a C-terminal Truncated Hepatitis C Virus Core Protein with Plasmid DNA Vaccine Leads to in vitro Assembly of Heterogeneous Virus-like ParticlesNelson Acosta-Rivero, Joanna Poutou, Alexis Mussachio et al.Recently, it has been shown that HCV core proteins (HCcAg) with C-terminal deletions assemble in vitro into virus-like particles (VLPs) in the presence of structured RNA molecules. Results presented in this work showed that a truncated HCcAg variant covering the first 120 aa (HCcAg.120) with a 32 aa N-terminal fusion peptide (6xHistag-XpressTMepitope) interacts with plasmid DNA vaccine. Interestingly, the buoyant … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,754 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHydrocarbon Biodegrading Potentials of a Proteus vulgaris Strain Isolated from Fish SamplesPatience O. Olajide, L. B. OgbeifunA Proteus vulgaris bacterium SR-1 was isolated from a freshly killed fish sample collected close to the point of crude oil spill in the Niger Delta region, Nigeria. Problem statement: The application of native bacterial species in bioremediation processes has long been desired, because they would be cost effective and efficient in terms of acclimation time. The ability to isolate … …American Journal of Applied Sciences, Vol. 7, Issue 7 · 31 Jul 2010 · 8,277 accessesView PDFCite