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14 results for “plant tissue culture”
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Literature ReviewOpen accessEvery word in the titleControl of Contamination of Tissue Plant Cultures During in Vitro Clonal MicropropagationIrina Anikina, Nursultan Kaynidenov, Dora Dayu Rahma Turista et al.Plant micropropagation is currently an important method for modern large-scale propagation of valuable plant crops. Among the risks limiting the success of this method is the contamination of cultured plants. Conventional surface sterilization methods are not always effective against internal infection of plant tissues, and secondary contamination of tissue cultures during replication is also a major problem. This causes scientific … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 8 Apr 2025 · 8,693 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCollection and Processing of Medicinal Raw Materials from Some Rare Plants in the Forest-Steppe Zone of Northern KazakhstanMarat Tynykulov, Svetlana Derbush, Marina Kuznetsova et al.Rare endemic plants of the forest-steppe zone of Northern Kazakhstan, including Adonis vernalis, Adonis wolgensis, Pulsatilla patens, and Pulsatilla multifida, represent valuable medicinal resources whose sustainable utilization requires optimized harvesting, processing, and conservation strategies. This study evaluated the effects of drying conditions, environmental factors, extraction techniques, and biotechnological approaches on the yield and quality of bioactive compounds from these species. … …OnLine Journal of Biological Sciences, Vol. 26, Issue 1 · 1 Apr 2026 · 1,595 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBasal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic EmbryogenesisJameel M. Al-KhayriProblem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 10,156 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake GrassWan Nurul Hidayah Wan Anuar, Suat-Hian Tan, Siti Hamidah Radiyah Shiekh Mahmud et al.Medicinal plants which is Clinacantus nutans (C. nutans) that known as Sabah Snake Grass or Belalai Gajah can treat several diseases including cancer. In order to provide and grow this plant in large number for health and pharmaceutical industry, one of the solutions is through the plant tissue culture technique. In this experiment, C. nutans was cultured under controlled conditions … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 8,193 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of in vitro Rooting through Growth Media, Gelling Agents and Activated Charcoal in Lycium chinenseJae Kwang Kim, Sang Un ParkLycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 26 Jun 2017 · 7,267 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in Rehmannia Glutinosa L.Aye Aye Thwe, Hyun Ho Kim, Haeng Hoon Kim, Sang Un ParkPlant tissue culture has proven to be commercially important for the replication of various plants species, including medicinal and ornamental plants. This technique has been used extensively in research for applications in agriculture and forestry. This study examines the effect of activated charcoal and the relative effectiveness of Gelrite compared to Phytagar. The number of roots per explant (8.2) and … …OnLine Journal of Biological Sciences, Vol. 15, Issue 2 · 12 Jun 2015 · 7,103 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L )Abdullatif AlkhateebThe effect of different concentration of date palm syrup (2, 4, 6, 8 and 10%) and sucrose at concentration of 30 and 60 g/l in addition to the control (without carbon source) on the micro propagation of date palm "cv. Suckary" were investigated. The results indicated that date syrup was taken up from the media as shown by the increase … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 1 · 31 Mar 2008 · 7,982 accessesView PDFCite
Research ArticleOpen accessIn the abstractResponse of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache RugosaWoo Tae Park, Thanislas Bastin Baskar, Sun Kyung Yeo et al.In this study a total of seven different Agrobacterium rhizogenes strains were evaluated for their ability to transform the plant Agastache rugosa and to produce the secondary metabolite rosmarinic acid. All the strains of A. rhizogenes i.e., 13333, 15834, A4, LBA9402, R1000, R1200 and R1601 strains tested here in this study, were able to induce hairy root formation in leaf … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 3 Sep 2017 · 7,866 accessesView PDFCite