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6 articles for “partial purification”
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Research ArticleOpen accessPhrase in the titleA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBiochemical Studies of Lipase from Germinating Oil Seeds (Glycine max)P. P. Gadge, S. D. Madhikar, J. N. Yewle et al.Problem statement: Lipase is one of the important enzymes in food, pharmaceutical, detergent and biofuels industries. Search for the lipase with distinct features, possibly from germinating seeds, is of interest for industrial applications. Approach: The lipase produced by soybean oil seeds was partially purified and characterized in terms of the optimal pH and temperature for activity as well as substrate … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 19 Jan 2012 · 9,703 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Antioxidant and Antibacterial Activities of the Polysaccharides from Mycelium and the Culture Medium of Pleurotus Eryngii Strain UN-1Qing Liu, Haodong Wu, Ying Wu et al.Pleurotus eryngii was one of the most precious and ordinary edible mushrooms, and its polysaccharides were the major active substances. In this study, P. eryngii strain UN-1 was cultured in a liquid medium at 25℃ under a shaking of 80 rpm for 7 days. Two crude polysaccharides named CPN and CPNM were obtained from the mycelium of P. eryngii UN-1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 19 Jul 2022 · 6,176 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces SpeciesFerdinand U. Paschaline, Onyeocha O. Ignatius, Ezeji E. Uchechukwu et al.The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarbon polluted soil and identified using standard microbiological and biochemical techniques. Fermentation was carried out for enzyme production and the extracellular extract was precipitated with 50% ammonium sulphate salts and further purified by gel filtrationusing sephadex … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 7 Dec 2019 · 7,398 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioautography Guided Identification of Anticandidal Compounds from A. terreus st. 1B. K. Nagaraja, R. Sripathy, C. N. Ramchand, M. SundararamanProblem statement: Saprophytic and soil inhabiting microorganisms are prolific producers of secondary metabolites with wide range of structural and functional diversity. The objective of the current study was to screen and evaluate the anti-candidal properties of soil inhabiting fungi. Approach: Preliminary dual culture assay and bioautography was used to screen the fungi with potential anti-fungal activity and direct detection of … …American Journal of Infectious Diseases, Vol. 7, Issue 4 · 1 Dec 2011 · 7,629 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordPurification of Natural Gas with Impurities using Membrane Processes: Parameter EstimationN. W. Jusoh, K. K. Lau, A. M. ShariffProblem statement: Membrane processes have received a significantly high attention on natural gas separation processes since this technology provides higher operating and cost advantages as compared to other technologies. One of the major problems related to natural gas separation using membrane is the existence of feed impurities including CO2, heavy hydrocarbon and water. Approach: Hence, mathematical model that is able … …American Journal of Engineering and Applied Sciences, Vol. 5, Issue 1 · 22 May 2012 · 8,426 accessesView PDFCite