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Research ArticleOpen accessEvery word in the titleAnalysis of Interferon-Gamma Release Assay Results for Latent Tuberculosis Infection Diagnosis at a Referral Clinical Laboratory in South KoreaTae Soung Kim, Ga Yeon Kim, Young Ki Lee, Jae Kyung KimThe world health organization states that individuals with Latent Tuberculosis Infection (LTBI) are infected with Tuberculosis (TB) bacteria but do not show symptoms of active disease. However, they are at possibility of developing vigorous TB and potentially transmitting the illness to others. In 2018, South Korea reported a 14.8% positivity rate in LTBI screening, with higher rates observed among older … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 4 · 25 Oct 2023 · 6,037 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn-Vivo Evaluation of Hexavalent Chromium Induced DNA Damage by Alkaline Comet Assay and Oxidative Stress in Catla catlaKantha Deivi Arunachalam, Sathesh Kumar Annamalai, Jaya Krishna KuruvaIn the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory … …American Journal of Environmental Sciences, Vol. 9, Issue 6 · 15 Jan 2014 · 7,200 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of enzyme Immunoassay with Immunofluorescence Assay for the Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections in ChildrenOsama Mohammed Saed Abdul-WahabMycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults. Currently, diagnosis of M. pneumoniae infection will based principally on serology and the detection of IgM can provide an early and sensitive diagnosis in children. A variety of commercial immunodiagnostic assays, such as Indirect Enzyme-Linked Immunosorbent (ELISA) assay and Indirect Immunofluorescence (IFA), are now available … …American Journal of Immunology, Vol. 9, Issue 2 · 21 Jun 2013 · 7,416 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiomarker Quantitation: Analytical Considerations for Ligand Binding Assay Regression Curves and Quality Control SamplesMark DysingerAs biomarkers grow in relevance for both the design and support of therapeutics and the clinical trials associated with them, there is an ever increasing need for accurate quantitation of these biochemical entities in biological matrices. While quantifying many biotherapeutics via ligand binding assay platforms can be fairly straightforward, biomarkers present some unique challenges that must be taken into account … …American Journal of Immunology, Vol. 8, Issue 3 · 25 Aug 2012 · 8,030 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleZinc Phosphide Induced DNA Damage in the Blood Cells of Gallus sp. using Comet DNA AssayK. A. Muid, R. M. Shahjahan, R. Begum, R. A. BegumProblem statement: The comet DNA assay is considered a suitable and rapid test for DNA-damaging potential in environmental and biomonitoring studies. Approach: During the study comets were analyzed as a measure of DNA damage to quantify the damage in the blood cells of fowls (Gallus sp.) after exposure to Zinc Phosphide (Zn3P2). Here the potential for DNA strand breaks by … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 1 · 3 Mar 2012 · 8,957 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparative Antioxidant Effects of Essential Oil Blends and Individual Oils Using the DPPH Radical-Scavenging AssaySunghun Jang, Jae Kyung KimOxidative stress is recognized as a major contributor to conditions such as cancer, aging, and inflammation. Consequently, there is a growing interest in the use of natural antioxidants, particularly essential oils, owing to their safety benefits and potent antioxidant properties. The aim of this study was to evaluate the antioxidant effects of four essential oil blends (Refresh, Zest, Revitalizing, and … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 3 · 5 Jan 2026 · 2,659 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssessment of Thyroid Hormone Level Alterations in Hepatitis B-Positive Serum Using a Quantitative Immunofluorescence Assay: A Randomized Controlled TrialHyeokjun Yun, Bo Kyeung Jung et al.This retrospective study aimed to assess the differences in serum biomarker levels, specifically thyroid hormone (free T4 and Thyroid-Stimulating Hormone [TSH]) levels, according to the presence or absence of Hepatitis B Virus (HBV) infection. Using a quantitative immunofluorescence assay, we analyzed serum samples from patients with confirmed HBV infection at Dankook University Hospital in Cheonan Province, Republic of Korea, between … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 31 Jul 2023 · 5,037 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEndotoxin Activity Assay: An Experience of a Southern-Italy HospitalLinda Degl’Innocenti, Flaviano Di Paola, Rizzo Marianna, Gerardino AmatoEndotoxin activity assay is a novel and rapid test capable of differentiating Gram-negative sepsis from infections carried by other bacteria. The test, moreover, represents a useful tool in the management of the septic patient. We show the data collected during a 6-year period experience, related to samples from septic patients admitted in ICU; for each patient we performed an EAA … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 5 Jan 2015 · 7,168 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssay for Rapid Screening of Phytochemicals as Antimicrobial AgentsGhosh Saurav, Indranil Mukherjee, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriThe present study aims to develop a rapid method for antibiotic sensitivity detection and screening of natural products for antimicrobial activity. The dimension of WBC in blood film was found to get altered when seeded with bacteria and monitored under light microscope. The shrinkage was prevented in response to antibiotic treatment and validated using statistical analysis (two sample one tailed … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 8,307 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,774 accessesView PDFCite