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8 results for “lysozyme”
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Research ArticleOpen accessEvery word in the titleIn Vitro Antimicrobial Activity of Modified Lysozyme (Lys 90) and Herbal Extracts Against Multidrug-Resistant Escherichia coliEni Kusumaningtyas, Bagem BR Sembiring, Andriani et al.The emergence of Antimicrobial Resistant (AMR) poses a significant challenge to the poultry farming industry. This study investigated the potential of Lysozyme (Lys), modified Lysozyme (Lys 90), an herbal extract, and their combination (herbs + Lys 90) as alternative antimicrobial agents against Multidrug-Resistant Escherichia coli (MDR-E. coli). Lys 90 was prepared by heating Lys at 90 °C for 20 minutes. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Dec 2025 · 2,076 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRaman Spectroscopy of Protein Crystal Nucleation and GrowthPechkova Eugenia, Maksimov, Georgy et al.Using Raman spectroscopy and the lysozyme as model system, we investigate the differences in protein conformation before and after Langmuir-Blodgett nanotemplate-induced crystal nucleation and growth. It was found, that the main difference in lysozyme conformation is associated to the higher amount of S-S bonds in lysozyme of LB crystals, probably in C-end of protein, resulting in the higher stiffness of … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 8 Dec 2014 · 8,275 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSynchrotron Powder Diffraction Study of Radiation Damage in Langmuir Blodgett Nanotemplate Crystallised ProteinJonathan P. Wright, Eugenia Pechkova, Claudio NicoliniPolycrystalline samples of lysozyme were prepared with and without a Langmuir-Blodgett (LB) thin film template via both the hanging drop method and batch crystallisation. Powder diffraction methods are used to compare these samples and to measure their resistance to radiation damage at room temperature. The X-ray induced amorphisation of the samples was followed as a function of time and it … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,175 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSCANNING ELECTRON MICROSCOPE IMAGING OF AMYLOID FIBRILSEisuke Takai, Gai Ohashi, Ryuichi Ueki et al.This study demonstrated the applicability of Scanning Electron Microscopy (SEM) for the observation of amyloid fibrils without staining. As model specimens, two types of amyloid fibrils with different shapes and chemical compositions were controllably synthesized from hen lysozyme. The apparent fibril widths in the SEM images were considerably larger than the original diameters analyzed by the conventional techniques of Transmission … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 1 Mar 2014 · 10,330 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIdentification and functional characterization of a novel lysine-rich protein from Entamoeba histolyticaAlka Mehra, Sudha Bhattacharya, Alok BhattacharyaIn an attempt to understand the role of genes with no known function, in the protozoan parasite Entamoeba histolytica, an analysis of the proteome was carried out and a Lysine-Rich protein (KRP) was identified for further study. This protein has a modular structure with four domains and was found to be expressed and localized to the cell surface of E. … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 4 · 31 Dec 2007 · 7,707 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Staphylococcus epidermidis S14 Screening Extracellular Antimicrobial MetabolitesJie Zhang, Han Jiang, Jiaxin Jiang, Guangrong HuangTotal 24 isolates with antimicrobial activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus and Shigella castellani, out of 259 strains, were isolated from the internal of seven marine fishes. The extracellular metabolites of strain S14, isolated from the internal of shark, showed stronger inhibition against the four tested bacteria, including G- and G+ bacteria. Based on the 16S rDNA the … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 19 Mar 2016 · 7,567 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Silico Characterization and Comparative Analysis of Allergenicity of Allergic Proteins from Different Food SourcesSourav Chakraborty, Shakhawat Hossain Foysal, Md. Nazmul Hasan, Nahian KhanAllergy is a steadily increasing health problem for all age groups. In general, it’s recommended that 10-35% of our daily calories come from protein. A complete protein source is one that provides all of the essential amino acids called high quality proteins. Although animal-based foods; for example, meat, poultry, fish, milk, eggs, are considered complete protein sources these are the … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 1 · 1 Dec 2014 · 7,360 accessesView PDFCite
Research ArticleOpen accessIn the abstractLANGMUIR-BLODGETT NANOTEMPLATE CRYSTALLIZATION COMBINED TO LASER-MICROFRAGMENTATION UNIQUELY CHARACTERIZE PROTEINS CRYSTALS BY SYNCHROTRON MICRODIFFRACTIONClaudio Nicolini, Luca Belmonte, Christian Riekel et al.Laser-induced microfragmentation of LB nanotemplate-induced protein crystals in glycerol solution results in distinct, coherently diffracting domains. Only crystals produced according to the Langmuir-Blodgett (LB) nanotemplate technique reveal in all four proteins being tested (lysozyme, insulin, thaumatin and ribonuclease) domains highly radiation resistant, while the crystals produced by the standard hanging drop crystallization method do not. Actually the very same laser … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 11 Feb 2014 · 7,984 accessesView PDFCite