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68 articles for “in vitro Analysis”
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Research ArticleOpen accessEvery word in the titleIn silico and in vitro Structural Analysis on the Interaction of Calmodulin and Calmodulin-Binding Motif of FKBP35 from Plasmodium knowlesiSazlinawatie Aladin, Cahyo Budiman et al.FK506-binding protein 35 (FKBP35) is a peptidyl-prolyl cis-trans isomerase found in Plasmodium knowlesi, a zoonotic malaria parasite responsible for concerning cases of malaria infection in East Malaysia. This protein contains a segment known as the calmodulin-binding motif (Pk-CBM), which is predicted to facilitate interaction with calmodulin from P. knowlesi (Pk-CaM). Although this interaction is considered promising for future antimalarial drug … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 10 Mar 2025 · 4,846 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAssessment of the Annona muricata Leaf Ethanol Extract Effect on The Diameter of Pancreatic Islets in Alloxan-Induced MiceSyafira N. Dewi, Supri I. Handayani, Marini Stephanie et al.Diabetes mellitus is one of a non-communicable disease whose prevalence continues to increase from year to year in both developed and developing countries. In Indonesia, it is estimated that 12 million people aged ≥15 years old had diabetes mellitus in 2013. Treatment of diabetes mellitus by using plant extract has been reported to have good results. One of the plants … …OnLine Journal of Biological Sciences, Vol. 20, Issue 1 · 9 Mar 2020 · 8,061 accessesView PDFCite
Research ArticleOpen accessIn the abstractOvocidal Effect of Achillea salicifolia and Hedysarum Gmelinii Extracts on Toxocara Cati Eggs in an in Vitro ExperimentLyudmila Lider, Yerlan Suleimen, Gulnur Mamytbekova et al.This study investigated the ovicidal activity of Achillea salicifolia and Hedysarum gmelinii extracts against Toxocara cati eggs under in vitro conditions. Two concentrations (5% and 10%) and four exposure times (0.5, 1, 24, 48 h) were tested in triplicate. The proportion of viable and non-viable eggs, embryo destruction, and shell degeneration were recorded microscopically. Statistical analysis was performed using one-way … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 19 Feb 2026 · 1,620 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Antimicrobial Activity of Modified Lysozyme (Lys 90) and Herbal Extracts Against Multidrug-Resistant Escherichia coliEni Kusumaningtyas, Bagem BR Sembiring, Andriani et al.The emergence of Antimicrobial Resistant (AMR) poses a significant challenge to the poultry farming industry. This study investigated the potential of Lysozyme (Lys), modified Lysozyme (Lys 90), an herbal extract, and their combination (herbs + Lys 90) as alternative antimicrobial agents against Multidrug-Resistant Escherichia coli (MDR-E. coli). Lys 90 was prepared by heating Lys at 90 °C for 20 minutes. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Dec 2025 · 2,076 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Kinematics Semen Tolerance to Oxidative StressCyril Mpho PilaneInterpretation of semen kinematic parameters remain as a point of discussion in predicting semen quality following processes like cryopreservation. During cryopreservation, oxidative stress remains a challenge in promoting post-thaw semen quality. Comparison of livestock semen exposed to oxidative stress, in vitro, highlight various aspects of sperm cell movement, like kinematics, to predict their qualities. Sperm cell kinematics includes the measurement … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 13 Sep 2024 · 4,560 accessesView PDFCite
Research ArticleOpen accessIn the abstractAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDFCite
Review ArticleOpen accessIn the abstractThe Impact of Di-2-Ethylhexyl Phthalate (DEHP) in Focus on the Reproductive System and Toxicity in Rat or Mammalian ModelVenkataramanaiah Poli, Srinivasulu Reddy MotireddyAbsalan, F., Saremy, S., Mansouri, E., Moghadam, M. T., Moghadam, A. R. E., & Razie, G. (2016). Effects of Mono-(2-Ethylhexyl) Phthalate and Di-(2-Ethylhexyl) Phthalate Administrations on Oocyte Meiotic Maturation, Apoptosis and Gene Quantification in Mouse Model. Cell Journal, 18(4), 503–513. https://doi.org/10.22074/cellj.2016.4717 Acevedo‐Rodriguez, A., Kauffman, A. S., Cherrington, B. D., Borges, C. S., Roepke, T. A., & Laconi, M. (2018). Emerging … …American Journal of Pharmacology and Toxicology, Vol. 19, Issue 1 · 6 Jun 2024 · 12,990 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Role of Ganoderma lucidum Polysaccharides Against Stress Induced by Heavy Metals in Caenorhabditis elegansHuixiu Ma, Dehui Dai, Guicai Chen, Weilian HuAli, H., Khan, E., & Ilahi, I. (2019). Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation. Journal of Chemistry, 2019, 6730305. https://doi.org/10.1155/2019/6730305 Aljerf, L., & Aljerf, N. (2023). Food products quality and nutrition in relation to public. Balancing health and disease. Progress in Nutrition, 25(1), e2023024. https://doi.org/10.23751/pn.v25i1.13928 Aljerf, L., Williams, M., Ajong, A. B., Onydinma, … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 18 May 2024 · 6,947 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic SeleniumMoh. Sofi’ul Anam, Andriyani Astuti, Budi Prasetyo Widyobroto et al.Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 26 Oct 2023 · 5,215 accessesView PDFCite