6 articles for “extraction protocols”

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Research ArticleOpen accessIn the keywordsMixed-Polarity Azeotropic Solvents for Efficient Extraction of Lipids from Nannochloropsis MicroalgaeRobert D. Long, Elseddik AbdelkaderProblem statement: The extraction of lipids from microalgae for biofuel production is a significant problem due to the intractability of their cell wall. The aim of this study of lipid extraction from Nannochloropsis microalgae was to examine the relative efficiencies of some commonly used solvent systems and test the use of co-boiling solvents in Soxhlet extraction as an improved method. … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 2 · 12 Aug 2011 · 9,387 accessesView PDF Cite

Review ArticleOpen accessIn the abstractTextual Figure Processing Method: A Systematic Literature ReviewWydyanto Yanto, Norshita Mat Nayan, Riza SulaimanA human transaction always involves figured documents that have high practical value and could be screened using Optical Character Recognition (OCR). Optical character recognition is a knowledge to process different types of documents and figures to become data that could be analyzed, stored in files, edited and written for different uses. Lately, researchers used a lot of methods to analyze … …American Journal of Applied Sciences, Vol. 21, Issue 1 · 26 Oct 2024 · 8,176 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case StudyJyoti Bhardwaj, Sudesh Kumar YadavThree protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 28 Oct 2013 · 7,925 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIsolation of Amylolytic Bacteria from Viscera of Labeo rohita (Ham.) and Optimization of Alpha Amylase ExtractionDebabrat Baishya, Hridip Kumar SarmaProblem statement: Fish is largely consumed in the north-eastern part of India resulting in the generation of huge amount of fish viscera in the market places. These waste generated is not properly managed for disposal. Under such circumstances it is necessary to properly utilize this waste regenerating the possibility of environment friendly disposal. Besides, industrially and commercially useful biological products … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 8,051 accessesView PDF Cite

Research ArticleOpen accessIn the abstractExtraction of Genomic DNA Using Magnetic Nanoparticles (Fe3O4) as a Solid-Phase SupportZ. M. Saiyed, C. N. RamchandMagnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 9,826 accessesView PDF Cite