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Research ArticleOpen accessIn the keywordsApplication of a Serum/Protein-Free Medium for the Growth of Mammalian Cell Lines and High Level Production of Classical, Variant and Very Virulent Strain of Infectious Bursal Disease Virus (IBDV)Ane Franciele Tapparo, Paloma Oliveira Tonietti, Maria CecÍlia R. Luvizotto, Tereza C. CardosoThe aim of this study was to verify the capacity of IBDV strains, Lukert, F52/70 and very virulent Brazilian genotype (G11) to infect and replicate on cell culture maintained without serum or protein supplementation. BHK21 and CER cells were directed adapted to growth under absence of protein and/or fetal bovine serum with addition of 5 µg mL-1 of insulin-like growth … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 7,656 accessesView PDFCite