15 results for “enzyme kinetics”

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Research ArticleOpen accessIn the keywordsNon-Target Inhibition of Antioxidant Enzymes in Honey Bees (A. mellifera and A. florea) Upon Pesticide ExposureAmal Ahmed Berjawi, Syed Salman Ashraf, Mohammad Ali Al-DeebUnderstanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 10 Apr 2020 · 7,394 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsTESTING CARBOXYMETHYL CELLULASE ACTIVITY SECRETED BY TRICHOPHYTON TERRESTRE IN CARBOXYMETHYL CELLULOSE SOLUTIONMansoor Ahmad Lone, Sanjay Sahay, Ravinder, DeepakThe techniques employed for bioconversion of cellulosic biomass to simple sugars by cellulases have a great industrial brunt. Cold-adapted microorganisms are potential resource of cold-active carboxymethyl cellulases secluded from the cold regions. In this study, Trichophyton terrestre-a rare fungal species in Indian soils, isolated from the rhizosphere of Juglans regia L. during winter season was subjugated for the production and … …Current Research in Microbiology, Vol. 5, Issue 1 · 4 Feb 2015 · 7,677 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsOn the Hormetic Behaviour of Drugs Binding to Different Redox States of Amine Oxidase EnzymesDeepak Narang, G. Reid McDonald, David J. Smith et al.Classical monoamine oxidases and copper-containing semicarbazide-sensitive amine oxidases are drug targets for a variety of established and novel pharmaceuticals used to treat conditions such as depression, Parkinsonism and inflammatory disorders. Development of enzyme inhibitors, both historically and currently, assumes an underlying adherence of these enzymes to Michaelis-Menten kinetic principles. In this mini-review, we discuss historical data from several laboratories and … …American Journal of Pharmacology and Toxicology, Vol. 3, Issue 1 · 31 Mar 2008 · 6,928 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCyclodextrin Glucanotransferase Activity of Microbial Strains Isolated from Yam-Cultivation Soils in Northwestern BeninCyrille Alode Vodounon, Ténor Dias-Mendel Allode et al.β-Cyclodextrin, an industrially important oligosaccharide, is produced by microbial enzymes such as cyclodextrin glucanotransferase (CGTase). This study investigated ways to optimize the enzymatic activity of CGTase from Bacillus cereus, Bacillus pumilus, Bacillus brevis, and Bacillus subtilis strains isolated from soils in north-western Benin. Cyclization activity of CGTase and the influence of pH, temperature, and metal ions were assessed using conventional … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 21 Sep 2026 · 652 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIn vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic SeleniumMoh. Sofi’ul Anam, Andriyani Astuti, Budi Prasetyo Widyobroto et al.Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 26 Oct 2023 · 5,215 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDF Cite

Research ArticleOpen accessIn the abstractUtilization of Banana, Pineapple and Watermelon Wastes-Substrate: As Consortiums to Remediating Cyanide Polluted SoilFlorence Obiageli Nduka, Samuel Chibuike Ubani, Victor Eshu Okpashi et al.The act of processing cassava for varied food products has come with unguided and wide-spread contamination of cyanide in the environment. The need for effective reduction in cost to enable remediation of cyanide-contaminated sites from cassava mill factories is the purpose of this investigation. This involves bio-stimulation of organic wastes with indigenous microorganisms to degrade cyanide. The application of organic … …American Journal of Environmental Sciences, Vol. 14, Issue 2 · 30 Apr 2018 · 9,930 accessesView PDF Cite

Research ArticleOpen accessIn the abstractOptimisation of Bromelain Enzyme Extraction from Pineapple (Ananas comosus) and Application in Process IndustryResmi Mohan, Venkatasubramanian Sivakumar, Thirumalaisamy Rangasamy, Chellappa MuralidharanBromelain is a general name for a family of sulfhydryl proteolytic enzymes obtained from Ananas comosus, the pineapple plant. The objective of the present study is to compare the activity of Bromelain from pineapple fruit and peel and to check the efficiency of this crude Bromelain enzyme in unhairing step in leather processing. The crude Bromelain was extracted and activity … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 19 Sep 2016 · 12,650 accessesView PDF Cite

EditorialOpen accessIn the abstractCommentary-How do we Improve Training for Receptor Research?Marilee Ann BenoreThis issue of the American Journal of Biochemistry and Biotechnology features articles about receptor-ligand interactions that are critical to the wellbeing of all living organisms. The importance of the field of receptor research is supported by the large number of articles published in journals, the numerous specialty journals, the international conferences and the percentage of legal drugs on the market … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 4 · 8 Dec 2015 · 8,056 accessesView PDF Cite

Research ArticleOpen accessIn the abstractReaction Kinetics of Versatile Peroxidase for the Degradation of Lignin CompoundsN. Busse, D. Wagner, M. Kraume, P. CzermakThe H2O2-dependent degradation of adlerol by a crude versatile peroxidase from Bjerkandera adusta, a new ligninolytic enzyme, was investigated. Adlerol (1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol)) is a non phenolic β-O-4 dimer whose structural architecture represents the most abundant unit (50-65%) of the valuable renewable biopolymer lignin. Lignin removel plays a key role in utilizing lignocellulosic biomass in biorefineries. Steady state analyses in the µL … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 9,263 accessesView PDF Cite