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412 articles for “enzyme”
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Research ArticleOpen accessEvery word in the titleImpact of Multi-Enzyme Machine Cleaning, Multi-Enzyme Soaking, and Traditional Tap Water Cleaning on the Cleanliness of Blood-Contaminated Hemostatic ForcepsLi Kang, Qingmin Gao, Yi FengEffective cleaning of surgical instruments is critical for infection prevention in healthcare settings. This study compared the cleaning efficacy, efficiency, and resource consumption of three methods, multi-enzyme machine cleaning (Group S1), multi-enzyme soaking (Group S2), and traditional tap water cleaning (Group S3), on blood-contaminated hemostatic forceps. A total of 600 blood-contaminated hemostatic forceps collected from the disinfection supply room of … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 24 Apr 2026 · 1,881 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUnveiling the Potential of Dombeya buettneri K.Schum As a Drug Agent: Molecular Docking of Compounds With Enzymes Linked to Diabetes and Neurodegenerative DiseaseEsther Emem Nwanna, Samuel Chima Ugbaja et al.This study investigated the mechanisms of Dombeya buettneri (snowball leafy) extracts on diabetes, hypertension, and neurodegenerative diseases. It compared extracts obtained using steep water (a by-product of corn wet milling) and aqueous water. The extracts were analyzed for total phenol, total flavonoid content, antioxidant activities such as FRAP, ABTS, NO, DPPH, iron-induced lipid peroxidation assay, degradation of deoxyribose (Fenton’s reaction), … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 1 Feb 2026 · 2,025 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of a Phytomineral Complex and an Enzyme Preparation on the Absorption of Nutrients and the Energy Balance of Calves' DietEvgeniia Erenko, Aleksandr Ovchinnikov, Marat Aubakirov et al.Cost-effective animal husbandry directly depends on the correct breeding of replacement young animals using diets corresponding to the feeding rate for all nutrition elements of the detailed system. Critical to these diets are biogenic elements, pivotal in enzymatic reactions and immune defense. However, specifics on enhancing nutrient absorption through dietary supplements remain underexplored, particularly in the context of calves' preweaning … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 12 Jun 2024 · 4,592 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlepH and Temperature Optimization of Several Bacterial Isolates from Mangrove Waters in the Mandeh Area to Produce Cellulase EnzymeFeskaharny Alamsjah, Dwiky Rahmadi Aswan, Anthoni AgustienEnzymes serve as vital biological catalysts, playing a crucial role in numerous industrial processes to accelerate specific biochemical reactions. In this study, we aimed to optimize cellulase enzyme production by investigating the influence of temperature and pH on four bacterial isolates: PUA-18, PUA-21, PUA-28, and PUA-38. The experiment was carried out in triplicates using a one-factor-at-a-time design, with pH and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 28 Mar 2024 · 6,476 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Amylase Activity by Modulating Abiotic Factors and Enzyme Stability in the Thermophilic Bacterial IsolatesAnthoni Agustien, Denny Bendrianis, Feskaharny Alamsyah et al.Amylase is one of the enzymes that is widely used in bioprocess technology, amylase from different microbial sources also varies in form, so it is suitable for its specific application. The purpose of this study was to determine the optimum temperature, pH, agitation, and stability of the amylase enzyme from two thermophilic bacterial isolates, TUA-07 and TUA-09. This study used … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 7 Feb 2024 · 5,493 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic SeleniumMoh. Sofi’ul Anam, Andriyani Astuti, Budi Prasetyo Widyobroto et al.Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 26 Oct 2023 · 5,215 accessesView PDFCite
Case ReportOpen accessEvery word in the titleEffects of Foliar Applications Mulberry Leaf Enzyme Solution Fertilizer on Growth and Quality of Hydroponic Chinese CabbageLi Yang, Fan Yuting, Du Qingjie et al.To investigate the effects of foliar applications mulberry leaf enzyme solution fertilizer on growth, nutritional quality, photosynthetic characteristics, mineral elements, and antioxidant enzyme system of hydroponic Chinese cabbage, Chinese cabbage Zheng bai'886'was used as experimental material. In this study, we mainly selected seven different fermentation periods (CK: Distilled water, T1-T6, the fermentation days were 20, 35, 50, 65, 80, and … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 7 Jan 2023 · 5,347 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Influence of Trichoderma viride Cellulase Enzyme Concentration on Saccharification of Waste Paper MaterialsKhomotso M. P. Mokatse, M. Alfred Mogale, J. Pieter H. Van WykEnzymatic hydrolysis of lignocellulosic materials is one of the major steps in the commercialization process of converting cellulosic substrates into bio-products. The saccharification of lignocellulosic materials is usually achieved by a synergistic action of an enzyme mixture consisting of multiple cellulase enzymes such as endo-and exo-glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. During the enzymatic hydrolysis of the … …OnLine Journal of Biological Sciences, Vol. 21, Issue 4 · 28 Oct 2021 · 6,456 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleNon-Target Inhibition of Antioxidant Enzymes in Honey Bees (A. mellifera and A. florea) Upon Pesticide ExposureAmal Ahmed Berjawi, Syed Salman Ashraf, Mohammad Ali Al-DeebUnderstanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 10 Apr 2020 · 7,394 accessesView PDFCite