5 results for “complementation”

Set a search alert

Research ArticleOpen accessEvery word in the titleGenetic Complementation Studies of Human Pin1 in Azotobacter vinelandii Revealed that it Requires Amino Terminus of the NifM to Deliver PPIase Effect to the Fe-protein of NitrogenaseKumaraguru Raja, Lakshmi Pulakat, Nara GaviniThe NifM is a peptidyl prolyl cis-trans isomerase and is required for the maturation and activation of the Fe protein of Nitrogenase. Since the carboxyl terminus of NifM is similar to the Human Pin1, we expressed the Human Pin1 in A. vinelandii BG98, a nifM mutant strain containing a kanamycin insertion and found that it could not complement the function … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,708 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsNifH: Structural and Mechanistic Similarities with Proteins Involved in Diverse Biological ProcessesSurobhi Lahiri, Lakshmi Pulakat, Nara GaviniThe NifH protein is a subunit of the nitrogenase enzyme that catalyzes the reduction of atmospheric nitrogen to ammonia. This protein contains highly conserved regions including the nucleotide binding sites, metal center ligands and the Switch I and Switch II domains. A number of proteins have structural and mechanistic similarities as well as evolutionary relationships with the NifH protein, notable … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 6,671 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDiscovery of Evolutionary Divergence of Biological Nitrogen Fixation and Photosynthesis: Fine Tuning of Biogenesis of the NifH and the ChlL by a Peptidyl-Prolyl Cis/Trans IsomeraseNara Gavini, Sinny Delacroix, Kelvin Harris Jr., Lakshmi PulakatProblem statement: Despite the structural and functional similarities between the nitrogenase that performs biological nitrogen fixation reaction and the Dark Protochlorphyllide Oxidoreductase (DPOR) that performs chlorophyll-biosynthesis, attempts to substitute nitrogenase-components with DPOR-components have hitherto failed. This investigation was undertaken to test if Chlamydomonas reinhardtii protochlorophyllide (Pchlide) reductase (ChlL) that shares some structural similarity with Nitrogenase Reductase (NifH) could complement the … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 17 Feb 2012 · 7,614 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPalm Oil Milling Wastes and Sustainable DevelopmentA.C. Er, Abd. Rahim Md. Nor, Katiman RostamProblem statement: Palm oil milling generates solid wastes, effluent and gaseous emissions. The aim of this study is to assess the progress made in waste management by the Malaysian palm oil milling sector towards the path of sustainable development. Sustainable development is defined as the utilization of renewable resources in harmony with ecological systems. Inclusive in this definition is the … …American Journal of Applied Sciences, Vol. 8, Issue 5 · 17 May 2011 · 7,691 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDeterministic Timed AFA: A New Class of Timed Alternating Finite AutomataAbdelaziz Fellah, Zachary Friggstad, Soufiane NoureddineTimed Alternating Finite Automata (TAFA), a natural generalization of Timed Finite Automata (TFA), are synchronous and powerful models for real-time computations. They become an effective and expressive model for developing embedded systems with real-time constraint computations which are required in many applications. We introduce Deterministic Timed Alternating Finite Automata (DTAFA), a new class of timed alternating finite automata, extended with … …Journal of Computer Science, Vol. 3, Issue 1 · 31 Jan 2007 · 7,499 accessesView PDF Cite