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22 results for “callus”
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Research ArticleOpen accessEvery word in the titleIn Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxiaBaan Munim Twaij, Alaa Jabbar Taha, Md. Nazmul HasanThe present study indicate the possibility of the development of callus from stem cut, leaf and root explants and regeneration of Datura plant (Datura innoxia) on Murashige and Skoog (MS) media supplemented with different concentrations of 6-Benzylaminopurine (BA) and 1-Naphthaleneacetic (NAA). Sterilized explants were inoculated and incubated in culture shade under different conditions (light and dark). Data analysis showed that … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 17 Aug 2019 · 8,367 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) CallusAli Akbar Ehsanpour, Roya RazavizadehWater stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,805 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInduction of Embryogenic Callus and Plant Regeneration in Carnation (Dianthus caryophyllus L.)Omid KaramiIn this study, efficient plant regeneration through somatic embryogenesis is achieved in four cultivars of carnation (Nelson, Sagres, Spirit and Impulse). Embryogenic calli were induced on petal explants only, all the calli established on leaf, sepal, receptacle and style explants were not embryogenic. Embryogenic calli were obtained on Morashige and Skoog basal medium (1962) containing sucrose 9%, 2.0 mg L-1 … …OnLine Journal of Biological Sciences, Vol. 8, Issue 4 · 31 Dec 2008 · 6,813 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCallus Induction and Cellular Suspensions from Murtilla (Ugni molinae Turcz.) for trans-resveratrol ProductionSalazar Carolina, Bustos Evelyn, Perez Claudia et al.The present study reports for the first time the quantification of resveratrol and the use of biotechnological techniques applied to stilbene productions in species from genus Ugni and calli production from adult explants (fruits). Resveratrol is synthesized by a series of families of higher plants, which has generated much interest in recent years for its antioxidant, anticancer and antitumor properties, … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 1 · 24 Jan 2017 · 7,942 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) GenotypesMd. Nazmul Hasan, Fahmid H. Bhuiyan, Hammadul Hoque et al.Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 19 Apr 2021 · 7,859 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBasal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic EmbryogenesisJameel M. Al-KhayriProblem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 10,156 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDFCite
Research ArticleOpen accessIn the keywordsControl of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud ExplantsSoubir Titov, Salil Kumar Bhowmik, Ajoy Mandal et al.The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl2 for 6 min. This study found that inflorescence tissues … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 3 · 30 Sep 2006 · 7,742 accessesView PDFCite
Research ArticleOpen accessIn the abstractSomatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) from Bud Explants Using Suspension CultureNurul Huda Panggabean, Isnaini Nurwahyuni et al.The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability to produce entire plants without the need for gamete fusion. While somatic embryo proliferation is typically performed on solid media, this approach is less efficient than liquid media, which is both more cost-effective and can lead to better proliferation results. … …OnLine Journal of Biological Sciences, Vol. 23, Issue 2 · 4 May 2023 · 5,863 accessesView PDFCite