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28 articles for “biochemical characterization”
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Research ArticleOpen accessPhrase in the titleHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCharacterization of Biochemical Compositions, Volatile Compounds and Sensory Profiles of Cabernet Sauvignon Wine in Successive Vintages (2008-2017)Yufeng Ding, Yanli Ma, Suping Li et al.Cabernet Sauvignon grape is an emblematic cultivar of Huailai region, China, allowing obtaining wines accounts for a large proportion of national consumption. This work aimed to determine the differences in basic parameters, organic acids, phenolics, volatile compounds and sensory profiles of Cabernet Sauvignon wines of successive vintages (2008-2017). The total organic acids content ranged from 7.03 to 42.33 g L-1, … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 3 · 17 Aug 2020 · 6,972 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCharacterization of Bacterial Strains Isolated Through Microbial Profiling of Urine SamplesPoulomi Nandy, A. R. Thakur, S. Ray ChaudhuriThe present study was conducted to determine the microbial profile in urine samples. Differential and selective chromogenic culture media were used for the rapid detection, identification and enumeration of urinary tract pathogens namely, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Proteus mirabilis. Urine samples of normal healthy individuals as well as patients with Urinary Tract Infection (UTI) … …OnLine Journal of Biological Sciences, Vol. 7, Issue 1 · 31 Dec 2007 · 7,556 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Role of Ganoderma lucidum Polysaccharides Against Stress Induced by Heavy Metals in Caenorhabditis elegansHuixiu Ma, Dehui Dai, Guicai Chen, Weilian HuAli, H., Khan, E., & Ilahi, I. (2019). Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation. Journal of Chemistry, 2019, 6730305. https://doi.org/10.1155/2019/6730305 Aljerf, L., & Aljerf, N. (2023). Food products quality and nutrition in relation to public. Balancing health and disease. Progress in Nutrition, 25(1), e2023024. https://doi.org/10.23751/pn.v25i1.13928 Aljerf, L., Williams, M., Ajong, A. B., Onydinma, … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 18 May 2024 · 6,947 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Superior Symbiotic Bacterial Isolates from the Oenggae Sea with Potential as AntibacterialLukas Pardosi, Adelya Irawan Manalu, Charles Venirius LisnahanSponges are one of the marine biota that have a symbiotic relationship with bacteria which can produce secondary metabolites as antimicrobials. The main aim of this research was to isolate and identify bacteria that had potential as antibacterials against multi-drug resistant test bacteria. The sponsor samples used in this research were Stylissa massa, Salvador Dali, and Angelas sp. The methods … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 26 Mar 2024 · 5,053 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces SpeciesFerdinand U. Paschaline, Onyeocha O. Ignatius, Ezeji E. Uchechukwu et al.The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarbon polluted soil and identified using standard microbiological and biochemical techniques. Fermentation was carried out for enzyme production and the extracellular extract was precipitated with 50% ammonium sulphate salts and further purified by gel filtrationusing sephadex … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 7 Dec 2019 · 7,398 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDFCite