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6 results for “agarose gel electrophoresis”

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Research ArticleOpen accessIn the keywordsCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity in Merozoite Surface Protein.1 of Plasmodium falciparum in Highlands: Wamena Papua IndonesiaRosye Hefmi Rechnelty Tanjung, Yulius Sarungu, Meidy Johana Imbiri et al.Malaria is the first killer as well as endemic disease in highland, lowland and coastal areas of Papua. In 2016 there were 160 thousands cases noted and 80% of the cases were caused by Plasmodium falciparum. Total sample for this study were provided by a clinical trial conducted from May to June 2018 in the regional hospital of Puncak Jaya, … …American Journal of Infectious Diseases, Vol. 14, Issue 4 · 11 Jan 2019 · 7,753 accessesView PDFCite
Research ArticleOpen accessIn the abstractMorpho-Physiological and Genetic Responses of Bali Local Rice Cultivars to Drought Stress at Seedling StageMade Pharmawati, Luh Putu WrasiatiRice is the main food source in many countries in the world. One of the major limiting factors for rice growth and productivity is drought stress. In Bali there are several local rice cultivars, two of them are ‘Mansur' and ‘Putih Cempaka'. The cultivar ‘IR 64' is one rice cultivar released by IRRI. The responses of those rice cultivars to … …OnLine Journal of Biological Sciences, Vol. 18, Issue 1 · 29 Mar 2018 · 7,644 accessesView PDFCite
Research ArticleOpen accessIn the abstractConservation of Binding of Llama and Other Animals’ Hemoglobins to Haptoglobins Across SpeciesMoges Woldemeskel, Oluseyi A. VanderpuyeThe plasma protein haptoglobin binds hemoglobin released from lysed erythrocytes. It causes removal of the free hemoglobin, thus preventing pathological oxidation of cells. Hemoglobin higher level structures are well conserved across animal species but primary structures can be as little as 50% homologous. Because of these differences, the question arose as to what extent hemoglobins can bind to haptoglobins from … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 26 Jan 2018 · 9,200 accessesView PDFCite
Research ArticleOpen accessIn the abstractLipoprotein (a) and Apolipoprotein (a) Isoforms in Patients with Acute Myocardial InfarctionAkram Saleh, Izzat AL-Awwaa, Sally Awwadeh, Nabil BashirProblem statement: The objective of this study is to determine the relationship between plasma lipoprotein (a) levels and apolipoprotein (a) isoforms in a group of Jordanian patients with Acute Myocardial Infarction (AMI) Approach: A total of 90 patients with acute myocardial infarction were compared with 90 age-and sex-matched controls. Lipoprotein (a) levels were measured by ELIZA method and isoforms were … …Current Research in Medicine, Vol. 1, Issue 1 · 30 Jun 2010 · 7,535 accessesView PDFCite
Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDFCite