Research ArticleOpen accessIn the author namesExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,987 accessesView PDFCite
Research ArticleOpen accessIn the author namesStudy on the Enhancement of Hypoglycemic and Antioxidant Activities of Compound Herbal Tea through Solid-State FermentationQing Liu, Xiaowen Que, Yueping Yang et al.This study utilized Aspergillus niger KY2295 for the Solid-State Fermentation (SSF) of a compound tea made from Cinnamomum cassia bark, Glycyrrhiza glabra, Morus abla L. leaf, and Crataegus oxyacantha L. to enhance its hypoglycemic effects. The fermentation process was optimized using single-factor experiments and a Central Composite Design (CCD), with α-amylase and α-glucosidase inhibitory activities as evaluation indices. Following fermentation, … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 20 May 2025 · 5,482 accessesView PDFCite
Research ArticleOpen accessIn the author namesDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,117 accessesView PDFCite