5 results for “Two-Dimensional Electrophoresis (2-DE)”

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Research ArticleOpen accessIn the keywordsA common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case StudyJyoti Bhardwaj, Sudesh Kumar YadavThree protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 28 Oct 2013 · 7,931 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsProteomics Approach for Investigating the Disease Resistance Using Cucumber as Model PlantSaligrama A. Deepak, Junko Shibato, Hideo Ishii et al.The profile of proteins induced in cucumber leaves pretreated with Acibenzolar-S-Methyl (ASM) and challenge inoculated with Colletotrichum orbiculare are presented. ASM was applied to the first leaf and the third leaf collected at different time intervals of 0-48 h and the total protein extracted using trichloroacetic acid acetone was analyzed by two-dimensional gel electrophoresis. Increased expressions of pyridoxine biosynthesis protein, … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 7,243 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsA Proteomics Analysis of Drought Stress-Responsive Proteins as Biomarker for Drought-Tolerant Sugarcane CultivarsNisachon Jangpromma, Supansa Kitthaisong, Khomsorn Lomthaisong et al.Problem statement: The prime objective in breeding selection process of drought-tolerant sugarcane is to identify the correlating marker, which could lead to rapid screening for drought-tolerant cultivars. In this study, we have reported an unknown 18-kDa protein (p18) along with other stress-inducible proteins to be highly expressed in sugarcane leaves under drought stress condition. Approach: The 2D-PAGE patterns of proteins … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 9,819 accessesView PDF Cite

Research ArticleOpen accessIn the abstract2-D Gel Electrophoresis Map of Methicillin-Resistant Staphylococcus aureus Treated with Quercus Infectoria Gall ExtractDayang Fredalina Basri, Lee Seng Aik, Radhiah Khairon, Mariati Abdul RahmanThe widespread outbreak of Methicillin-Resistant Staphylococcus Aureus (MRSA) has caused clinical and epidemiological concern in hospital environment. The emergence of Vancomycin-Intermediate S. Aureus (VISA) and, more recently, Vancomycin-Resistant S. Aureus (VRSA) has further alarmed clinician and scientist worlwide. The objective of this study is to determine the optimum concentration of sample protein from MRSA after treatment with acetone extract from … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 7,998 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Recursive Application of a Support Vector Machine for Protein Spot Detection in 2-Dimensional Gel ElectrophoresisGary D. Boetticher, Hisham Al-Mubaid, Karen Frasier-ScottTwo-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis remains the core of proteomic technology because it is currently the most powerful method to analyze large collections of proteins. Advances in electrophoresis equipment are making this technique more accessible but effective computer assisted protein spot detection remains a very labor-intensive endeavor. Protein spot analysis is still time consuming, requires human intervention and … …Journal of Computer Science, Vol. 1, Issue 3 · 30 Sep 2005 · 6,678 accessesView PDF Cite