5 results for “Transfection”

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InvestigationOpen accessIn the keywordsDevelopment of Genetic Constructions for Exctocytosis ControlIrina Nikolaevna Dominova, Vitaly Anvarovich Kasymov, Ekaterina Vladimirovna Silina et al.A fragment of the research project devoted to the development of genetic control of exocytosis is presented in the work, which can further ensure the success of targeted therapy of various diseases, including neurodegenerative ones. For the purpose of specific transfection of intracellular cascades in astrocytes in vivo, we have developed an experimental sample of the reagent kit. The latter … …OnLine Journal of Biological Sciences, Vol. 18, Issue 2 · 29 Jan 2018 · 7,310 accessesView PDF Cite

Review ArticleOpen accessIn the keywordsDNA Vaccination Associated with Eliminating Inheritance Risks during Embryo Development: A ReviewRia MargianaThe process of immunization using modified DNA is essential whenever scientists desire to trigger an artificial response in the host’s body to improve immunity. The use of DNA vaccines awakens the somatic cells of the host to produce antigens that will assist the body to prepare to fight infections through preventive measures triggered by the vaccines. These biological inventions have … …OnLine Journal of Biological Sciences, Vol. 16, Issue 2 · 26 May 2016 · 6,873 accessesView PDF Cite

EditorialOpen accessIn the abstractCRISPR-CAS SYSTEM: A FEASIBLE SOLUTION FOR GETTING RID OF PERSISTENT VIRAL INFECTION?Zhaohua ZhongMany viruses, including DNA and RNA viruses, can persistently replicate in the infected cells and cause a prolonged damage. Herpes viruses can be latent in its host cells. Human Immunodeficiency Virus (HIV) reversely transcripts its RNA genome to DNA and integrates to the host’s chromosome. Hepatitis B Virus (HBV) and Human Papilloma Virus (HPV) persistently stay in the host cells … …Current Research in Virology, Vol. 3, Issue 1 · 10 Nov 2014 · 12,614 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProduction of Recombinant Proteins in Insect CellsChristian Kollewe, Andreas VilcinskasAmong the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression Vector System (BEVS). We discuss the different strategies … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 13,742 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCocaine Exposure Results in Formation of Dendritic Varicosity in Rat Primary Hippocampal NeuronsHonghong Yao, Crystal Bethel-Brown, Anil Kumar, Shilpa BuchProblem statement: It has been well documented that drugs of abuse such as cocaine can cause enhanced progression of HIV-Associated Neuropathological Disorders (HAND), the underlying mechanisms mediating these effects remain poorly understood. Approach: In present study, we explored the impact of cocaine exposure (I and 10 μM) on the dendritic beading in rat primary hippocampal neurons. Using the approach of … …American Journal of Infectious Diseases, Vol. 5, Issue 1 · 31 Mar 2009 · 7,889 accessesView PDF Cite