40 results for “Tissue Culture”

Set a search alert

Research ArticleOpen accessPhrase in the titleSeedling Propagation of Kulo Chrysanthemum by Tissue CultureJeany Polii MandangAims of this research were to get the right dosage of Plant Growth Regulkator (PGR) and Coconut Water (CW) for shoot multiplication of Kulo chrysanthemums. The study was conducted using a factorial design of three factors namely IAA (0.0; 0.5 and 1.0 ppm), BAP (0, 0.5 and 1.0 ppm) and coconut water (0.0 and 30%). The explants used were clean … …American Journal of Agricultural and Biological Sciences, Vol. 12, Issue 3 · 6 Nov 2017 · 8,179 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleMorphogenesis in Tissue Culture of Local Kazakh Cotton VarietiesBishimbayeva Nazira, Yertayeva Bakhyt, Amirova Aigul, Rakhimbayev IzbasarCallus was initiated from hypocotyl and cotyledon explants of seven of commercial Kazakh cotton cultivars on MS medium supplemented with 0,1 mg L-1 2,4-D and 0,5 mg L-1 kinetin. The percentage of callus formation ranged from 64,0 to 91,0% for cotyledons and from 72 to 90% for hypocotyls. Study of morphological heterogeneity peculiarities of metamorphosis and histological structure of calli … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 12 Jul 2017 · 7,171 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleGenetic Relationship among Wild Medicinal Genotypes of Ziziphora canescens Benth. and Ziziphora tenuior L. and Detection of Genetic Variations Resulted From Tissue Culture, Salinity and pH MediaAbdulkarim Dakah, Mohamad Suleiman, Salim ZaidZiziphora tenuior L. and Ziziphora canescens Benth. are two of plants which using in folk medicine in the Kalamoon Mountains areas of Syria for cough, stomachache and dysentery. Samples of Ziziphora genotypes were collected from four different locations (Assal Al-Ward, Yabroud, Qarah and Maaraba), 20 RAPD and 12 ISSR primers were used to assess the genetic diversity of 10 genotypes. … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 3 · 19 Oct 2015 · 7,897 accessesView PDF Cite

Literature ReviewOpen accessEvery word in the titleControl of Contamination of Tissue Plant Cultures During in Vitro Clonal MicropropagationIrina Anikina, Nursultan Kaynidenov, Dora Dayu Rahma Turista et al.Plant micropropagation is currently an important method for modern large-scale propagation of valuable plant crops. Among the risks limiting the success of this method is the contamination of cultured plants. Conventional surface sterilization methods are not always effective against internal infection of plant tissues, and secondary contamination of tissue cultures during replication is also a major problem. This causes scientific … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 8 Apr 2025 · 8,693 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsCollection and Processing of Medicinal Raw Materials from Some Rare Plants in the Forest-Steppe Zone of Northern KazakhstanMarat Tynykulov, Svetlana Derbush, Marina Kuznetsova et al.Rare endemic plants of the forest-steppe zone of Northern Kazakhstan, including Adonis vernalis, Adonis wolgensis, Pulsatilla patens, and Pulsatilla multifida, represent valuable medicinal resources whose sustainable utilization requires optimized harvesting, processing, and conservation strategies. This study evaluated the effects of drying conditions, environmental factors, extraction techniques, and biotechnological approaches on the yield and quality of bioactive compounds from these species. … …OnLine Journal of Biological Sciences, Vol. 26, Issue 1 · 1 Apr 2026 · 1,595 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsSomatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) from Bud Explants Using Suspension CultureNurul Huda Panggabean, Isnaini Nurwahyuni et al.The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability to produce entire plants without the need for gamete fusion. While somatic embryo proliferation is typically performed on solid media, this approach is less efficient than liquid media, which is both more cost-effective and can lead to better proliferation results. … …OnLine Journal of Biological Sciences, Vol. 23, Issue 2 · 4 May 2023 · 5,863 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEffect of Serial Passaging on the Morphology and Biological Characteristics of Human Adipose Tissue-derived Stem CellsLenka Oravcova, Martin Bohac, Lubica Krajciova et al.Somatic stem cells posses an exclusive property of self regeneration and plasticity which makes them promising candidates for use in tissue engineering and regenerative medicine. In the case of stem cell therapy it is crucial to obtain them in sufficient amount. That's why they have to be cultured under in vitro conditions. Long term culture provides adequate number of cells, … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 17 Dec 2016 · 7,242 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsIn Vitro Prevention of Browning in Plantain CultureInnocent Chimereze Onuoha, Chinonye Jemimah Eze, Chibuikem I.N. UnambaProblem statement: The in vitro propagation of plantain (Musa paradisiaca) is still faced with lots of challenges such as blackening or browning of tissues prior to culture due to the oxidation of phenolic compound by polyphenolic oxidase enzyme present in the tissue when excised. Approach: Understanding browning processes in plantain and possible ways of minimizing it during excision of explants … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 15,229 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsComparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L )Abdullatif AlkhateebThe effect of different concentration of date palm syrup (2, 4, 6, 8 and 10%) and sucrose at concentration of 30 and 60 g/l in addition to the control (without carbon source) on the micro propagation of date palm "cv. Suckary" were investigated. The results indicated that date syrup was taken up from the media as shown by the increase … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 1 · 31 Mar 2008 · 7,982 accessesView PDF Cite