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Research ArticleOpen accessEvery word in the titleOptimizing Boar Epididymal Sperm Preservation: Comparative Analysis of Slicing Float-Up vs. Flushing and BTS vs. Andromed® Extenders on Sperm Motility and Morphometry TraitsMamonene Angelinah Thema, Ntuthuko Raphael Mkhize, Gerhard van der Horst et al.Epididymal sperm collection represents a valuable strategy for preserving genetics from deceased or castrated boars of superior breeding value. The two primary collection methods-slicing float-up (high sperm yield) and flushing (low blood contamination risk)-differ in sperm recovery efficiency, while semen extenders provide cryoprotective nutrients that protect against cold shock during preservation. This study compared the effects of slicing float-up versus … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 14 Mar 2026 · 1,794 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimalization of the Swim-Up Method for Bali Bull Sperm: Effect of Incubation Time and Medium on Sperm KineticsAthhar Manabi Diansyah, Muhammad Yusuf, Rahmat et al.Bali bulls (Bos javanicus) are an essential part of Indonesia’s indigenous livestock, prized for their ability to thrive in challenging environments, their natural disease resistance, and their strong reproductive capabilities. The aim of this study was to refine the swim-up technique for preparing Bali cattle sperm. We evaluated the effects of two commonly used media Tyrode's Albumin Lactate Pyruvate (TALP) … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 9 Feb 2026 · 1,687 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleQuality of Sperm Simmental Bulls and Success of Artificial Insemination with the Addition of Nanocalcium Phosphate in Tris Aminomethane Egg Yolk Diluent Using Semen Storage Ampoules from Nanocalcium Silicophosphate BiomaterialsEnike Dwi Kusumawati, I Wayan Karyasa, Yogi Pratama Putra et al.Success in Artificial Insemination (AI) is largely determined by the quality of the stored semen, therefore storage devices, especially straws or ampoules, are an important part that cannot be separated from the overall semen cryopreservation technology. Making a prototype of semen storage ampoules from nanocalcium silicophosphate biomaterials is based on the need for straws or ampoules. Semen storage in spermatozoa … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 2 · 28 May 2024 · 5,211 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen MethodsEnike Dwi Kusumawati, N. Isnaini, A.P.A. Yekti et al.Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 2 · 9 May 2019 · 9,016 accessesView PDFCite
Review ArticleOpen accessEvery word in the titleSpermatogonial Stem Cells: A Current Update in the Management of Azoospermic Infertile MenSilvia W Lestari, Debby AdityaOne of the management of azoospermia is utilizing Assisted Reproductive Technologies (ART) by Intra-Cytoplasmic Sperm Injection (ICSI) with sperm obtained from the epididymis or directly from testis. In some conditions such as hypogonadism or undescended testis, the sperm are not successfully obtained. Thus, it is considered to perform Spermatogonial Stem Cells (SSCs) culture to restore men fertility. The objective of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 5 Dec 2017 · 6,923 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSperm DNA Fragmentation and Apoptosis Levels: A Comparison of the Swim up and the Density Gradient Centrifugation Methods for Sperm PreparationSilvia W. Lestari, Triyana Sari, Dwi A. PujiantoThis study investigated the efficiency of two sperm preparation methods, namely, density gradient centrifugation and the swim-up method, in selecting spermatozoa based on the DNA fragmentation index and the apoptosis levels. Fifteen semen samples from infertile couples underwent sperm preparation in this study. The Makler® counting chamber and Eosin Y staining were used to analyse the sperm concentration, motility and … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 14 Nov 2016 · 7,419 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSelenium and Cadmium Tissue Concentrations and the CASA Sperm Motility Analysis after Administration to RatsRobert Toman, Svatoslav Hluchy, Peter Massanyi et al.The accumulation of Selenium (Se) and Cadmium (Cd) and their effects on sperm motility parameters in rats were investigated. Male rats were dosed with Cd (group A: 2 mg kg-1 b.w., intraperitoneally, group B: 30 mg L-1, per os in drinking water) and Se (group D: 2 mg kg-1 b.w., intraperitoneally, group E: 5 mg L-1, per os in drinking … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 13 Dec 2014 · 7,979 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRole of Endometrial Thickness on Intracytoplasmic Sperm InjectionMohammed Al-SunaidiProblem statement: Endometrial receptivity is a key factor in successful outcome of in vitro fertilization. The endometrial receptivity is determined clinically by estimation of endometrial thickness and pattern by ultrasonography. This study was performed to evaluate the overall effect of endometrial thickness on pregnancy rate, multiple pregnancy rate and miscarriage rate in infertile women undergoing Intracytoplasmic Sperm Injection (ICSI) cycles … …American Journal of Applied Sciences, Vol. 9, Issue 4 · 13 Feb 2012 · 7,709 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Age and Postmortem Time on Some White-Tailed Deer (Odocoileus virginianus texanus) Epididymal Sperm Characteristics and Response of CryopreservationJ. Ake-Lopez, E. Cavazos-Arizpe, J. G. Magana-Monforte et al.Problem statement: Males rather than females of the White-Tailed deer (Odocoileus virginianus texanus) are more susceptible to hunting because their physical characteristics, therefore their elimination can increase a genetic degradation and a lower productivity because of the effect of gender. Thus, the aim of the current study was to evaluate the effect of age and postmortem time of White-Tailed deer … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 7,623 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRelation of Serum and Semen Malondialdehyde and Total Anti-Oxidants with Sperm Parameters in Infertile MenFirozeh Akbari-Asbagh, Ebrahim Mostafavi, Kobra Hamdi et al.Problem statement: Oxidative Stress (OS) has been recognized as one of the most important cause of male infertility. We studied the relation of serum and Semen Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) with sperm parameters in infertile men with sperm count within the normal range. Approach: The prospective case- control study performed on infertile men presenting to the infertility … …American Journal of Immunology, Vol. 6, Issue 3 · 13 Sep 2010 · 14,527 accessesView PDFCite