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14 articles for “Semen Extender”
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Research ArticleOpen accessPhrase in the titleUse of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid DamageClara Gonzalez-Marin, Michael Eduard Kjelland, Rosa Roy et al.Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 29 Dec 2012 · 7,430 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEvaluation of Japanese Quail Egg-Yolk Extender in Cryopreservation of Cockerel SemenRantloko Rolly Maapola, Khathutshelo Agree Nephawe, Tshimangadzo Lucky Nedambale et al.The purpose of this study was to examine the effects of quail egg yolk extenders in the cryopreservation of cockerel semen. Ten Venda cockerels were chosen randomly and semen was collected twice a week with a two-day rest period using an abdominal massage technique. Immediately following semen collection, semen samples were evaluated for microscopic parameters (sperm cell motility, viability, morphology, … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 25 Mar 2023 · 5,638 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of the Tensioactive Agent SDS on Extender’s Boar Semen Viability after ThawingMaria Helena Moreira da Silva, Loide Valadão, Fernando Moreira da SilvaEffects of the surfactant Sodium Dodecyl Sulfate (SDS) on the motility and viability of boar semen after thawing were evaluated. For such purpose, semen of twelve adult boars was collected twice a week, the rich portion of this semen was separated and frozen with different concentrations of SDS (0; 2.5; 5; 10; 20 and 40 mM). For freezing the semen … …American Journal of Applied Sciences, Vol. 18, Issue 1 · 20 Jan 2021 · 7,795 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOptimizing Boar Epididymal Sperm Preservation: Comparative Analysis of Slicing Float-Up vs. Flushing and BTS vs. Andromed® Extenders on Sperm Motility and Morphometry TraitsMamonene Angelinah Thema, Ntuthuko Raphael Mkhize, Gerhard van der Horst et al.Epididymal sperm collection represents a valuable strategy for preserving genetics from deceased or castrated boars of superior breeding value. The two primary collection methods-slicing float-up (high sperm yield) and flushing (low blood contamination risk)-differ in sperm recovery efficiency, while semen extenders provide cryoprotective nutrients that protect against cold shock during preservation. This study compared the effects of slicing float-up versus … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 14 Mar 2026 · 1,794 accessesView PDFCite
Research ArticleOpen accessIn the abstractSupplementation with Different Concentrations of Dithiothreitol and Glutathione During Cryopreservation of Semen from Large White BoarsMahlatsana Ramaesela Ledwaba, Masindi Lottus Mphaphathi et al.The study aims to compare different concentrations (0, 2.5, 5, 7.5 and 10 mM) of antioxidants, Dithiothreitol (DTT) and Glutathione (GSH), on sperm parameters following the freeze-thawing of semen from large white boars. Three large white boars were selected for semen collection and the collected semen was sent to the lab for analysis. Following dilution with Beltsville thawing solution and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 3 Jun 2024 · 5,517 accessesView PDFCite
Research ArticleOpen accessIn the abstractFrozen Semen Quality of Kolbroek Boars Concerning Glutathione ConcentrationsLerato Deirdre Sehlabela, Masindi Lottus Mphaphathi et al.Glutathione (GSH) is an antioxidant that is being studied for its ability to improvecellular responses to semen preservation challenges. The research goal aimed toevaluate the impact of introducing different glutathioneconcentrations to boar semen freezing extender (0, 1, 5, and 10 mM) onthe Kolbroek boar sperm's quality after being frozen and thawed. For 2 h, thecollected sperm-rich fraction was chilled at … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 1 Apr 2023 · 6,005 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy the Efficacy of Different Concentrations of Coconut Water on Boar Semen Following Equilibration at 18°C for Different HoursMduduzi M. Tshabalala, Masindi L. Mphaphathi, Thivhilaheli R. Netshirovha et al.A rich natural resourcesuch as Coconut Water (CCW) features high concentrations of antioxidants,nutrients, amino acids, sugars, and electrolytes. These herbal extractinfusions are used in semen extenders to preserve animal sperm. In addition,CCW is important for in vitro productionof embryos. The purpose of this study was to investigate the use of coconutwater as an alternative bulking agent for boar semen after … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 4 · 23 Nov 2022 · 7,040 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen MethodsEnike Dwi Kusumawati, N. Isnaini, A.P.A. Yekti et al.Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 2 · 9 May 2019 · 9,016 accessesView PDFCite