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17 articles for “Semen Cryopreservation”
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Research ArticleOpen accessEvery word in the titleEvaluation of Japanese Quail Egg-Yolk Extender in Cryopreservation of Cockerel SemenRantloko Rolly Maapola, Khathutshelo Agree Nephawe, Tshimangadzo Lucky Nedambale et al.The purpose of this study was to examine the effects of quail egg yolk extenders in the cryopreservation of cockerel semen. Ten Venda cockerels were chosen randomly and semen was collected twice a week with a two-day rest period using an abdominal massage technique. Immediately following semen collection, semen samples were evaluated for microscopic parameters (sperm cell motility, viability, morphology, … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 25 Mar 2023 · 5,638 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSupplementation with Different Concentrations of Dithiothreitol and Glutathione During Cryopreservation of Semen from Large White BoarsMahlatsana Ramaesela Ledwaba, Masindi Lottus Mphaphathi et al.The study aims to compare different concentrations (0, 2.5, 5, 7.5 and 10 mM) of antioxidants, Dithiothreitol (DTT) and Glutathione (GSH), on sperm parameters following the freeze-thawing of semen from large white boars. Three large white boars were selected for semen collection and the collected semen was sent to the lab for analysis. Following dilution with Beltsville thawing solution and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 3 Jun 2024 · 5,517 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDFCite
Research ArticleOpen accessIn the keywordsFrozen Semen Quality of Kolbroek Boars Concerning Glutathione ConcentrationsLerato Deirdre Sehlabela, Masindi Lottus Mphaphathi et al.Glutathione (GSH) is an antioxidant that is being studied for its ability to improvecellular responses to semen preservation challenges. The research goal aimed toevaluate the impact of introducing different glutathioneconcentrations to boar semen freezing extender (0, 1, 5, and 10 mM) onthe Kolbroek boar sperm's quality after being frozen and thawed. For 2 h, thecollected sperm-rich fraction was chilled at … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 1 Apr 2023 · 6,005 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAssociation Between Climatic Factors with Fresh Semen Quality Parameters in Madura Cattle (an Indonesian Native Breed)Nurul Isnaini, Bela Dwi Wadi Lestari, Faizal Andri et al.Semen quality is a critical point to ensure the success of the artificial insemination program. One factor which highly determine the semen quality of bulls is climatic conditions. The purpose of the present study was to evaluate the association between climatic factors and fresh semen quality parameters in Madura bulls. A total of 1583 ejaculates which were collected from 5 … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 15 Sep 2021 · 6,810 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSeasonal Variations in Semen Quality of Pasundan Bulls in their Native Tropical EnvironmentNurul Isnaini, Tri Harsi, Anggit Damaratri Lapoliwa et al.The success of artificial insemination highly depends on the semen quality, in which it may significantly associate with the environmental factor. However, the information regarding the seasonal effects on semen quality of Pasundan bulls is still scarce. This study aimed to examine seasonal variation in semen quality of Pasundan bulls in their native environment. A total of 203 ejaculates produced … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 2 · 27 Apr 2021 · 7,931 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Different Freezing Curves on the Acrosome Integrity of Male Goat Sperm CellsSofia Alexandra Fagundes Lopes, Henrique José Duarte Rosa, António Chaveiro, Fernando Moreira da SilvaThe aim of the present study was to evaluate the effect of three different freezing curves on male goat’s sperm viability and acrosome integrity. Semen was collected on three adult male goats in February (Azores, Portugal, latitude 38°39′N) using an artificial vagina and a female with induced heat. Three collection sessions were spaced of approximately 5 days. The study followed … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 19 Feb 2021 · 6,616 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDFCite
Research ArticleOpen accessIn the keywordsKinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of Eurycoma longifolia Jack Aqueous ExtractFalah Hasan Baiee, Abd Wahid Haron, Rosnina Haji Yusoff et al.This study was carried out to improve the kinetic motilities of frozen-thawed bull semen diluted with tris-based egg yolk diluent that was supplemented with Eurycoma longifolia jack aqueous extract. A total of 24 ejaculates were obtained from six cross-bred bulls using an electro-ejaculator. The extract of Eurycoma longifolia jack was distributed into three low doses and three high doses; cryopreserved … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 16 Jun 2017 · 8,635 accessesView PDFCite
Research ArticleOpen accessIn the abstractImproving the Technology for Assessing the Semen of Beige Karakul SheepAigerim Turekyzy Bigarayeva, Nuradin Alibayev, Arkadiy Kanurovich Natyrov et al.The article presents the research results on the effective use of seed products from breeding rams in reproduction for the conservation and targeted propagation of genetic resources of Karakul sheep of rare beige color, which are on the verge of extinction. Selected freshly obtained ejaculates from 6 beige rams of the Karakul breed in the amount of 32 samples, meeting … …OnLine Journal of Biological Sciences, Vol. 25, Issue 1 · 17 Feb 2025 · 4,501 accessesView PDFCite